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Team:Chiba/jk/β
From 2008.igem.org
(Difference between revisions)
(→Week 2) |
(→Week 1) |
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| Line 5: | Line 5: | ||
*17 August, 2008 | *17 August, 2008 | ||
| - | :Transforation | + | :[[Team:Chiba/protocol/transformation|Transforation]] |
::*[http://partsregistry.org/Part:BBa_R0040| BBa_R0040](2008) -> No colonies on plates | ::*[http://partsregistry.org/Part:BBa_R0040| BBa_R0040](2008) -> No colonies on plates | ||
::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2008) -> No colonies on plates | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2008) -> No colonies on plates | ||
| Line 15: | Line 15: | ||
*18 August, 2008 | *18 August, 2008 | ||
| - | : | + | ::[[Team:Chiba/protocol/transformation|Transforation]] |
::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) | ||
::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) | ||
| Line 29: | Line 29: | ||
*19 August, 2008 | *19 August, 2008 | ||
:[[Team:Chiba/protocol/PCR|PCR]] | :[[Team:Chiba/protocol/PCR|PCR]] | ||
| - | :: | + | ::*[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007) |
| - | :: | + | ::*[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006) |
| - | :: | + | ::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007) |
| - | :: | + | ::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006) |
| - | :: | + | ::*[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007) |
| - | :: | + | ::*[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006) |
| - | :: | + | ::*[http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)･･･control |
:[[Team:Chiba/protocol/gelcheck|Gel Check]] | :[[Team:Chiba/protocol/gelcheck|Gel Check]] | ||
| - | :: | + | ::*[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007) |
| + | ::*[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006) | ||
| + | ::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007) | ||
| + | ::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006) | ||
| + | ::*[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007) | ||
| + | ::*[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006) | ||
| + | ::*[http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007) | ||
*20 August, 2008 | *20 August, 2008 | ||
| - | :[[Team:Chiba/protocol/DNA Purification/sigma| | + | :[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]] |
::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) | ||
::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) | ||
| Line 55: | Line 61: | ||
:[[Team:Chiba/protocol/gelcheck|Gel Check]] | :[[Team:Chiba/protocol/gelcheck|Gel Check]] | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007) | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) | ||
*21 August, 2008 | *21 August, 2008 | ||
| Line 63: | Line 74: | ||
::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) | ::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) | ||
::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) | ::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) | ||
| + | |||
| + | :[[Team:Chiba/protocol/gelcheck|Gel Check]] | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007) -> None | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> None | ||
| + | |||
| + | |||
| + | :[[Team:Chiba/protocol/transformation|Transforation]] | ||
| + | (Locate & 2 Punch) | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates | ||
| + | ::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates | ||
| + | |||
| + | |||
| + | |||
| + | *22 August, 2008 | ||
| + | |||
| + | *23 August, 2008 | ||
==Week 2== | ==Week 2== | ||
Revision as of 13:20, 30 August 2008
β班の実験ノート
Contents |
Week 1
- 17 August, 2008
- Transforation
- [http://partsregistry.org/Part:BBa_R0040| BBa_R0040](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0079| BBa_C0079](2008) -> No colonies on plates
- 18 August, 2008
- Transforation
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2006) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
- Transforation
- 19 August, 2008
- PCR
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
- [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
- [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)･･･control
- Gel Check
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
- [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
- [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)
- 20 August, 2008
- Mini prep
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
- Digestion
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
- Gel Check
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
- 21 August, 2008
- PCR
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
- Gel Check
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007) -> None
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> None
(Locate & 2 Punch)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
- 22 August, 2008
- 23 August, 2008
Week 2
- 27 August, 2008
- Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate([http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)).
Week 3
reference
- L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone.FEMS Microbiol Lett 266 (2007) 1-9
- クオラムセンシングのクロストークについてのミニレビュー。クオラムセンシングを利用したAHLバイオセンサーについて、Table 1.にまとまっている。
- M.K Winson et al./FEMS Microbiology Letters 163 (1998) 185-192
- Figure 2.hは、LuxR、RhlR、LasRそれぞれの、C4-C14のN-acyl-L-homoserine lactone,C4-C14のN-(3-oxoacyl)-L-homoserine lactoneに対する感受性を比較したグラフ。
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