Team:LCG-UNAM-Mexico/Notebook/2008-July

From 2008.igem.org

(Difference between revisions)
Line 140: Line 140:
         </tr>
         </tr>
         <tr>
         <tr>
-
<td class="bodyText"><p>July 9th(summary)- -- -- -- -- -- -- --  - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ---- --- --- -- - ------ -- ---- --- --- --- -- -- ----- ---, --- - - --- -- -- -- -- -- -- --  - ------ -----.
+
<td class="bodyText"><p>Today  we discussed the following with Miguel:</p>
-
  --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ----. </p>
+
            <p>BIOPARTS:<br>
-
        </td>
+
              There  was information in regards to the transformation of the bioparts. Chiba’s team  explained to us that their biopart’s DNA was not in the registry however it was  mistakenly set as available. However they assured us that they have the functional bioparts, so we will have to request some DNA directly to the team.</p>
-
      </tr>  
+
            <p>ELECTRODES:<br>
 +
              Miguel thinks that measuring a conductivity change in the medium will be sufficient. The temporary plan is to create a document gathering everything we know on the  subject and that everyone helps investigating as much as possible.            </p>
 +
            <p>PARAMETERS  FOR THE MODEL:<br>
 +
              This  is further explained below, in modeling session, but basically what Miguel told us is  that we can’t count with experimental data for the moment. He suggested that we  should search for average half-lives and protein kinetics in general and use those parameters as a first approach. </p>
 +
            <p>CONTROLS:<br>
 +
              Now that the experimental work has inevitably been delayed, that time can be  invested in defining controls, both positive and negative for the  experiment.  </p></td>
 +
        </tr>  
<tr>
<tr>
           <td class="subHeader" bgcolor="#99CC66" id="10">2008-07-10</td>  
           <td class="subHeader" bgcolor="#99CC66" id="10">2008-07-10</td>  

Revision as of 20:05, 31 August 2008

LCG-UNAM-MexicoTeam

Header image
iGEM 2008 TEAM
line decor
  
line decor

 
 
 
 
 
 
July

July's summary:--- ---- - --- ---

2008-07-02

July 2nd(summary)- -- -- -- -- -- -- -- - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ---- --- --- -- - ------ -- ---- --- --- --- -- -- ----- ---, --- - - --- -- -- -- -- -- -- -- - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ----.

2008-07-03

July 3rd(summary)- -- -- -- -- -- -- -- - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ---- --- --- -- - ------ -- ---- --- --- --- -- -- ----- ---, --- - - --- -- -- -- -- -- -- -- - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ----.

2008-07-04

MODELING:
We defined our preliminary model’s assumptions and our equations with their respective stoichiometric matrix. The current assumptions and equations can be found in the Modeling section.

NOTES: 
- (d/dt)x=Ʃ(entering flows) - Ʃ(exiting flows)
- Law of mass action: The flow is equal to the reaction constant times the product of the mass of the reactants.

TO-DO LIST:
-Start doing simulations with Gepasi, Copasi or Matlab.
-Investigate the underlying dynamic of each equation (Law of Mass Action, Hill, Michaelis-Menten, etc.)
-Read the articles on our webpage, especially Adam Arkin’s and the one regarding lambda phage.

2008-07-08

MODELING:

MISSING REACTION:
We forgot to take into account one reaction, cI*’s degradation (cI* -» 0). Now it has been added to the model.

ASSUMPTIONS:
There were some changes in our assumptions.

CHECK:
- It is suggested to check more in-depth if nickel natural usage in the cell can really be considered irrelevant for our experiment (assumption 5).
 - Daniela identified the metabolic pathways which involve nickel in E. coli, the link is on assumption 5.

MODEL:
The first simulation in Matlab was generated. It worked! Everyone should test it and correct or suggest anything necessary.

TO-DO LIST:
Defining parameters is our topmost priority (reaction rates, kinetic constants and concentrations).

2008-07-09

Today we discussed the following with Miguel:

BIOPARTS:
There was information in regards to the transformation of the bioparts. Chiba’s team explained to us that their biopart’s DNA was not in the registry however it was mistakenly set as available. However they assured us that they have the functional bioparts, so we will have to request some DNA directly to the team.

ELECTRODES:
Miguel thinks that measuring a conductivity change in the medium will be sufficient. The temporary plan is to create a document gathering everything we know on the subject and that everyone helps investigating as much as possible.

PARAMETERS FOR THE MODEL:
This is further explained below, in modeling session, but basically what Miguel told us is that we can’t count with experimental data for the moment. He suggested that we should search for average half-lives and protein kinetics in general and use those parameters as a first approach.

CONTROLS:
Now that the experimental work has inevitably been delayed, that time can be invested in defining controls, both positive and negative for the experiment. 

2008-07-10

July 10th(summary)- -- -- -- -- -- -- -- - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ---- --- --- -- - ------ -- ---- --- --- --- -- -- ----- ---, --- - - --- -- -- -- -- -- -- -- - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ----.

2008-07-15

July 15th(summary)- -- -- -- -- -- -- -- - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ---- --- --- -- - ------ -- ---- --- --- --- -- -- ----- ---, --- - - --- -- -- -- -- -- -- -- - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ----.

2008-07-17

July 17th(summary)- -- -- -- -- -- -- -- - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ---- --- --- -- - ------ -- ---- --- --- --- -- -- ----- ---, --- - - --- -- -- -- -- -- -- -- - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ----.

2008-07-22

July 22nd(summary)- -- -- -- -- -- -- -- - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ---- --- --- -- - ------ -- ---- --- --- --- -- -- ----- ---, --- - - --- -- -- -- -- -- -- -- - ------ -----. --- ------ --- ---- -- --- ----- -- -- -- ------ ---- ---- - -- - ---- - ----- ---- ---, - ----- --- ----- ---- ---- ---- -- --- --- --- --- -- ---- ---------- - ------ ---- --- ---- ----- --- --- ----- ---- ----- ------ ---- -- -- --- ------- - ----- --- ----.