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Team:LCG-UNAM-Mexico/Notebook/2008-July
From 2008.igem.org
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<p>CHECK:<br> | <p>CHECK:<br> | ||
- It is suggested to check more in-depth if nickel natural usage in the cell can really be considered irrelevant for our experiment (assumption 5). <br> | - It is suggested to check more in-depth if nickel natural usage in the cell can really be considered irrelevant for our experiment (assumption 5). <br> | ||
| - | + | ||
| + | - Daniela identified the metabolic pathways which involve nickel in <em>E. col</em>,. Here is the <a href="http://biocyc.org/ECOLI/NEW-IMAGE?type=COMPOUND&object=NI%2b2">link</a>.</p> | ||
<p>MODEL:<br> | <p>MODEL:<br> | ||
The first simulation in Matlab was generated. It worked! Everyone should test it and correct or suggest anything necessary.</p> | The first simulation in Matlab was generated. It worked! Everyone should test it and correct or suggest anything necessary.</p> | ||
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<tr> | <tr> | ||
<td class="bodyText"><p>Today we discussed the following with Miguel:</p> | <td class="bodyText"><p>Today we discussed the following with Miguel:</p> | ||
| - | <p>BIOPARTS:<br> | + | <p><strong>BIOPARTS:</strong><br> |
There was information in regards to the transformation of the bioparts. Chiba’s team explained to us that their biopart’s DNA was not in the registry however it was mistakenly set as available. However they assured us that they have the functional bioparts, so we will have to request some DNA directly to the team.</p> | There was information in regards to the transformation of the bioparts. Chiba’s team explained to us that their biopart’s DNA was not in the registry however it was mistakenly set as available. However they assured us that they have the functional bioparts, so we will have to request some DNA directly to the team.</p> | ||
| - | <p>ELECTRODES:<br> | + | <p><strong>ELECTRODES:</strong><br> |
Miguel thinks that measuring a conductivity change in the medium will be sufficient. The temporary plan is to create a document gathering everything we know on the subject and that everyone helps investigating as much as possible. </p> | Miguel thinks that measuring a conductivity change in the medium will be sufficient. The temporary plan is to create a document gathering everything we know on the subject and that everyone helps investigating as much as possible. </p> | ||
| - | <p>PARAMETERS FOR THE MODEL:<br> | + | <p><strong>PARAMETERS FOR THE MODEL:</strong><br> |
This is further explained below, in modeling session, but basically what Miguel told us is that we can’t count with experimental data for the moment. He suggested that we should search for average half-lives and protein kinetics in general and use those parameters as a first approach. </p> | This is further explained below, in modeling session, but basically what Miguel told us is that we can’t count with experimental data for the moment. He suggested that we should search for average half-lives and protein kinetics in general and use those parameters as a first approach. </p> | ||
| - | <p>CONTROLS:<br> | + | <p><strong>CONTROLS:</strong><br> |
Now that the experimental work has inevitably been delayed, that time can be invested in defining controls, both positive and negative for the experiment. </p><br> | Now that the experimental work has inevitably been delayed, that time can be invested in defining controls, both positive and negative for the experiment. </p><br> | ||
<p><strong>MODELING:</strong><br> | <p><strong>MODELING:</strong><br> | ||
Revision as of 20:29, 31 August 2008
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