Team:Hawaii/Meeting/2008-06- 5

(Difference between revisions)

Revision as of 02:48, 6 June 2008

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9am St. John 515

Update progress on experiments performed this week
1. competent cell creation (taken 2 days), and testing results
Part B proposals
1. Grace
2. Krystle
3. Margaret (teleconference in from US mainland)

Present: GK, KS, MR, SC, GP, KS, NW, LG Presentations: Grace: lux operon night light

Discussion Points

rbc may be too strong a promoter for use in the proposed construct
test the strength of RBC and explore if it is leaky by creating a lacZ/GFP fusion protein

use the fusion protein plasmid from SC, use amplifier to get the RBCL promoter, cut out the fusion site and put into pCC1383. -> 'It can be made into a biobrick if it works.'

expression of lux as controlled by lac may not be enough without available lactose

- question: Is lactose membrane permeable for S. PCC6803? 'yes'
- may need to find a membrane permeable substance that can bind to the lac promoter

Krystle: bacterial export system, soluble proteins

Discussion Points

it will be best to focus on pilA and slr2016, signal sequences that have already been experimentally confirmed

Margaret: synthetic plasmid biobricks

Discussion Points

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 11: / Line 11: @@
 == Minutes ==
+Present: GK, KS, MR, SC, GP, KS, NW, LG
+Presentations:
+Grace: lux operon night light
+: <b>Discussion Points</b>
+:*rbc may be too strong a promoter for use in the proposed construct
+:*test the strength of RBC and explore if it is leaky by creating a lacZ/GFP fusion protein
+::*use the fusion protein plasmid from SC, use amplifier to get the RBCL promoter, cut out the fusion site and put into pCC1383. -> 'It can be made into a biobrick if it works.'
+:*expression of lux as controlled by lac may not be enough without available lactose
+::**question: Is lactose membrane permeable for S. PCC6803?  'yes'
+::**may need to find a membrane permeable substance that can bind to the lac promoter
+Krystle: bacterial export system, soluble proteins
+: <b>Discussion Points</b>
+:*it will be best to focus on pilA and slr2016, signal sequences that have already been experimentally confirmed
-Present: &lt;Person A&gt;, &lt;Person B&gt;
+Margaret: synthetic plasmid biobricks
+: <b>Discussion Points</b>
-#
 == Action Items ==