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User:University of Washington/10 September 2008
From 2008.igem.org
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[[UW Glycerol Stocks| Glycerol Stocks]] of TrbA, KorA, TrbC | [[UW Glycerol Stocks| Glycerol Stocks]] of TrbA, KorA, TrbC | ||
| + | |||
| + | ==LuxR from AraC and TetR(Faifan)== | ||
| + | |||
| + | -made 4 electro-competent MG1655Z1 from overnight x4 | ||
| + | |||
| + | -re-ligated pSB3K3 + promo + LuxR | ||
| + | *mix 8.58ul LuxR + 2ul T4Buffer + 5ul pSB3K3 + 3.42ul promo + 1ul T4 Ligase | ||
| + | |||
| + | -transformed + inoculated onto Kan plates | ||
| + | *5ul old LuxR construction ligation | ||
| + | *5ul new LuxR construction ligation | ||
| + | *5ul miniprep of LuxR colony2.7 (contains matching P1010) | ||
| + | *control: no plasmid, grew on Tsy plate | ||
---- | ---- | ||
Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 22:22, 10 September 2008
Glycerol Stocks of TrbA, KorA, TrbC
LuxR from AraC and TetR(Faifan)
-made 4 electro-competent MG1655Z1 from overnight x4
-re-ligated pSB3K3 + promo + LuxR
- mix 8.58ul LuxR + 2ul T4Buffer + 5ul pSB3K3 + 3.42ul promo + 1ul T4 Ligase
-transformed + inoculated onto Kan plates
- 5ul old LuxR construction ligation
- 5ul new LuxR construction ligation
- 5ul miniprep of LuxR colony2.7 (contains matching P1010)
- control: no plasmid, grew on Tsy plate
Back to Team:University_of_Washington/Notebook#Notebook
