Team:Hawaii/Notebook/2008-09-12

From 2008.igem.org

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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of transformants=== :<strong> Grace</strong> [[Image:091208colonyPCR.jpg|right|thumb|400px|EtBr stained 2%...)
(Made competent DH5α cells)
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:*Colony PCR of 3A, plac+rbs transformants
:*Colony PCR of 3A, plac+rbs transformants
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===Made competent DH5&alpha; cells===
 
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:<strong> Grace & Margaret</strong>
 

Revision as of 02:29, 13 September 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Verification of transformants

Grace
EtBr stained 2% agarose gel ran at 95V for 1 hour. Two microliters of PCR product were loaded into each well.
Construct Colony forming units
3A: nir+rbs + slr1 4
3A: nir+ rbs + pilA 32
3A: nir+rbs + slr1+GFPf 35
3A: nir+rbs + pilA+GFPf (8/12) 42
3A: nir+rbs + pilA+GFPf (8/13) 24
pSB1A3 (-) 25
plac+rbs + slr1 >300
plac+rbs + pilA >300
plac+rbs + slr1+GFPf >300
plac+rbs + pilA+GFPf (8/12) >300
plac+rbs + pilA+GFPf (8/13) >300
plac+rbs (-) 295
nir+rbs + slr1 >300
nir+rbs + pilA >300
nir+rbs + slr1+GFPf >300
nir+rbs + pilA+GFPf (8/12) >300
nir+rbs + pilA+GFPf (8/13) >300
nir+rbs (-) 301
  • Colony PCR of 3A, plac+rbs transformants



Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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