Team:Hawaii/Notebook/2008-09-12
From 2008.igem.org
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Revision as of 02:29, 13 September 2008
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
Construct | Colony forming units |
---|---|
3A: nir+rbs + slr1 | 4 |
3A: nir+ rbs + pilA | 32 |
3A: nir+rbs + slr1+GFPf | 35 |
3A: nir+rbs + pilA+GFPf (8/12) | 42 |
3A: nir+rbs + pilA+GFPf (8/13) | 24 |
pSB1A3 (-) | 25 |
plac+rbs + slr1 | >300 |
plac+rbs + pilA | >300 |
plac+rbs + slr1+GFPf | >300 |
plac+rbs + pilA+GFPf (8/12) | >300 |
plac+rbs + pilA+GFPf (8/13) | >300 |
plac+rbs (-) | 295 |
nir+rbs + slr1 | >300 |
nir+rbs + pilA | >300 |
nir+rbs + slr1+GFPf | >300 |
nir+rbs + pilA+GFPf (8/12) | >300 |
nir+rbs + pilA+GFPf (8/13) | >300 |
nir+rbs (-) | 301 |
- Colony PCR of 3A, plac+rbs transformants
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]