Team:Chiba/jk/β/weak 1

From 2008.igem.org

(Difference between revisions)

Revision as of 08:46, 25 September 2008

>送受信班

Week 1

17 August, 2008

Transformation

competent cells : XL10G

BBa_R0040(2008) [http://partsregistry.org/Part:BBa_R0040] -> No colonies on plates
BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] -> No colonies on plates
BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] -> No colonies on plates

18 August, 2008

PCR

BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076]
BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077]
BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078]
BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079]
BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control

DNA Template	1
dNTP mix	10
Foward Primer	5
Reverse Primer	5
DNA polymerase	1
Thermopol Buffer	10
dH2O	68
TOTAL	100μL

95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃

--->Gel Check

Sample DNA	3
Loading Dye	2
dH2O	7
TOTAL	12μL

From left;

BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] ->None
BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] ->None
BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] ->None
BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] ->None
BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] ->None
BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control ->OK

Transformation

competent cells : XL10G

BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
BBa_C0076(2006) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates(19/8)
BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]

--->(20/8)Mini prep

19 August, 2008

PCR

BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154]
BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154]
BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control

DNA Template	1
dNTP mix	10
Foward Primer	5
Reverse Primer	5
DNA polymerase VENT	1
Thermopol Buffer	5
dH2O	28
TOTAL	50μL

95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃

--->Gel Check

Sample DNA	3
Loading Dye	2
dH2O	7
TOTAL	12μL

From left;

BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] ->None
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] ->None
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] ->None
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] ->None
BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154] ->None
BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154] ->None
BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control ->OK

20 August, 2008

(18/8)--->Mini prep

BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]

--->Gel Check

Sample DNA	3
Loading Dye	2
dH2O	7
TOTAL	12μL

From left;

BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None

Sample DNA	10
Loading Dye	4
dH2O	10
TOTAL	24μL

From left;

BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> OK
BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK

Sample DNA	3
Loading Dye	2
dH2O	7
TOTAL	12μL

From left;

BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK

--->Digestion test

BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]

Sample No	1	2	3	4	5	6	7	8
BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]	3	-	-	-	3	-	-	-
BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]	-	3	-	-	-	3	-	-
BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]	-	-	3	-	-	-	3	-
BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]	-	-	-	3	-	-	-	3
EcoRⅠ	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1
PstⅠ	-	-	-	-	0.1	0.1	0.1	0.1
Buffer EcoRⅠ	1	1	1	1	1	1	1	1
BSA	1	1	1	1	1	1	1	1
dH2O	4.9	4.9	4.9	4.9	4.8	4.8	4.8	4.8
TOTAL	10	10	10	10	10	10	10	10

--->Gel Check

Sample DNA	3
Loading Dye	2
dH2O	7
TOTAL	12μL

From left;

(Single Digestion 1~4)

BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK

(Double Digestion 1~4)

BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK

21 August, 2008

(20/8)--->PCR

BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]

DNA Template	1
dNTP mix	10
Foward Primer	5
Reverse Primer	5
DNA polymerase TAQ	1
Thermopol Buffer	5
dH2O	28
TOTAL	50μL

95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃

--->Gel Check

Sample DNA	3
Loading Dye	2
dH2O	7
TOTAL	12μL

From left;

BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK

Transformation

competent cells : XL10G

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]

--->(23/8)Digestion

22 August, 2008

Transformation

competent cells : XL10G

BBa_J13002(2007) [http://partsregistry.org/Part:BBa_J13002]
BBa_J13002(2006) [http://partsregistry.org/Part:BBa_J13002]
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
BBa_J04500(2006) [http://partsregistry.org/Part:BBa_J04500]

(Locate & 2 Punch ~iyama ver.~)

BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
BBa_J04500(2008) [http://partsregistry.org/Part:BBa_J04500] -> No colonies on plates

--->(24/8)Mini prep

23 August, 2008

(21/8)--->Mini prep

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]

--->Digestion test

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]

Sample No	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-
BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]	-	-	-	-	-	-	1	-	-	-	-	-	-	3	-	-	-	-	-	-	3
EcoRⅠ	-	-	-	-	-	-	-	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1
PstⅠ	-	-	-	-	-	-	-	-	-	-	-	-	-	-	0.1	0.1	0.1	0.1	0.1	0.1	0.1
Buffer EcoRⅠ	-	-	-	-	-	-	-	0.9	0.9	0.9	0.9	0.9	0.9	0.9	0.8	0.8	0.8	0.8	0.8	0.8	0.8
BSA	-	-	-	-	-	-	-	1	1	1	1	1	1	1	1	1	1	1	1	1	1
dH2O	-	-	-	-	-	-	-	5	5	5	5	5	5	5	5	5	5	5	5	5	5
TOTAL	3	3	3	3	3	3	1	10	10	10	10	10	10	10	10	10	10	10	10	10	10

--->Gel Check

Sample No	1~6	7
Sample DNA	3	1
Loading Dye	1	1
dH2O	2	4
TOTAL	6	6

From left; Sanple No. 1~7

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]

Sample No	8~21
Sample DNA	10
Loading Dye	2
TOTAL	12

From left;

(Single Digestion) Sanple No. 8~14

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK

(Double Digestion) Sample No. 15~21

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK?

Digestion test

BBa_J04500(2007:old-sample)[http://partsregistry.org/Part:BBa_J04500]

Sample No	1	2	3
Sample DNA	3	3	3
EcoRⅠ	-	0.1	0.1
SpeⅠ	-	-	0.1
EcoRⅠ Buffer	-	0.9	0.8
BSA	-	1	1
dH2O	-	5	5
TOTAL	3	10	10

--->Gel Check

Sample No	1	2	3
Sample DNA	3	10	10
Loading Dye	1	2	2
dH2O	2	-	-
TOTAL	6	12	12

From left;

BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion -> OK
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad

>next week

ホーム	メンバー紹介	プロジェクト紹介	Parts Submitted to the Registry	モデリング	ノート

@@ Line 1: / Line 1: @@
->[[Team:Chiba/jk/γ|出力班]]
+>[[Team:Chiba/jk/β|送受信班]]
 ==Week 1==
+===17 August, 2008===
+:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
+:competent cells : XL10G
+::*BBa_R0040(2008) [http://partsregistry.org/Part:BBa_R0040] -> No colonies on plates
+::*BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] -> No colonies on plates
+::*BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
+::*BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
+::*BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
+::*BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] -> No colonies on plates
-==17,August 2008==
+===18 August, 2008===
-<br>PCR
+:'''[[Team:Chiba/protocol/PCR|PCR]]'''
-<br>BBa_E0430①
+::*BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070]
-<br>BBa_E0420②
+::*BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076]
-<br>BBa_J06702③
+::*BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077]
-<br>BBa_S01416④
+::*BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078]
-<br>BBa_I73003⑤
+::*BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079]
-<br>BBa_I73004⑥
+::*BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control
-<br>BBa_I730026⑦
-<br>BBa_I763002⑧
-<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">DNA Template</td>
+<td>1</td>
+</tr>
+<tr>
+<td>dNTP mix</td>
+<td>10</td>
+</tr>
+<tr>
+<td>Foward Primer</td>
+<td>5</td>
+</tr>
+<tr>
+<td>Reverse Primer</td>
+<td>5</td>
+</tr>
+<tr>
+<td>DNA polymerase</td>
+<td>1</td>
+</tr>
+<tr>
+<td>Thermopol Buffer</td>
+<td>10</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>68</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>100μL</td>
+</tr>
+</table>
+:95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
+:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+{|align="justify"
+|[[Image:Chiba-0818.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>3</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>2</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>7</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>12μL</td>
+</tr>
+</table>
+:From left;
+::*BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] ->None
+::*BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] ->None
+::*BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] ->None
+::*BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] ->None
+::*BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] ->None
+::*BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control ->OK
+|}
+:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
+competent cells : XL10G
+::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
+::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
+::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
+::*BBa_C0076(2006) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates(19/8)
+::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
+::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
+::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
+::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
+::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
+::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
+:--->(20/8)[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]
+===19 August, 2008===
+:'''[[Team:Chiba/protocol/PCR|PCR]]'''
+::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
+::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
+::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
+::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
+::*BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154]
+::*BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154]
+::*BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control
+<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">DNA Template</td>
+<td>1</td>
+</tr>
+<tr>
+<td>dNTP mix</td>
+<td>10</td>
+</tr>
+<tr>
+<td>Foward Primer</td>
+<td>5</td>
+</tr>
+<tr>
+<td>Reverse Primer</td>
+<td>5</td>
+</tr>
+<tr>
+<td>DNA polymerase VENT</td>
+<td>1</td>
+</tr>
+<tr>
+<td>Thermopol Buffer</td>
+<td>5</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>28</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>50μL</td>
+</tr>
+</table>
+:95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
+:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+{|align="justify"
+|[[Image:Chiba-0819.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>3</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>2</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>7</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>12μL</td>
+</tr>
+</table>
+:From left;
+::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] ->None
+::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] ->None
+::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] ->None
+::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] ->None
+::*BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154] ->None
+::*BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154] ->None
+::*BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control  ->OK
+|}
+===20 August, 2008===
+:(18/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
+::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
+::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
+::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
+::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
+::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
+::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
+::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
+::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
+::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
+:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+{|align="justify"
+|[[Image:Chiba-0820-3.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>3</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>2</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>7</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>12μL</td>
+</tr>
+</table>
+:From left;
+::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
+::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
+::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
+|}
+{|align="justify"
+|[[Image:Chiba-0820-4.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>10</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>4</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>10</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>24μL</td>
+</tr>
+</table>
+:From left;
+::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
+::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> OK
+::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
+::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
+::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK
+|}
+{|align="justify"
+|[[Image:Chiba-0820.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>3</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>2</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>7</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>12μL</td>
+</tr>
+</table>
+:From left;<br>
+::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK<br>
+::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK<br>
+::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK<br>
+::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK<br>
+|}
+--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
+::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
+::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
+::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
+::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
+<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
 <td width="257">Sample No</td>
-<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td><td>⑦</td><td>⑧</td>
+<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td>
 </tr>
 <tr>
-<td>DNA tamplate</td>
+<td>BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]</td>
-<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td><td>1</td><td>1</td><td>1</td>
+<td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td>
 </tr>
 <tr>
-<td>FW primer</td>
+<td>BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]</td>
-<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
+<td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td>
 </tr>
 <tr>
-<td>VR primer</td>
+<td>BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]</td>
-<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
+<td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td>
 </tr>
 <tr>
-<td>dNTPmix</td>
+<td>BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]</td>
-<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+<td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td>
 </tr>
 <tr>
-<td>thermo pol buffer</td>
+<td>EcoRⅠ</td>
-<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+<td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 </tr>
 <tr>
-<td>DNA pol(VENT)</td>
+<td>PstⅠ</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
+</tr>
+<tr>
+<td>Buffer EcoRⅠ</td>
+<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+</tr>
+<tr>
+<td>BSA</td>
 <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 </tr>
 <tr>
 <td>dH2O</td>
-<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
+<td>4.9</td><td>4.9</td><td>4.9</td><td>4.9</td><td>4.8</td><td>4.8</td><td>4.8</td><td>4.8</td>
 </tr>
 <tr>
 <td>TOTAL</td>
-<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
+<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
 </tr>
 </table>
@@ Line 55: / Line 365: @@
-==20,August 2008==
+:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
-<br>TRANSFORMATION
+{|align="justify"
-<br>BBa_J52008(Luciferase Renilla)-->colony
+|[[Image:Chiba-0820-2.JPG]]
-<br>BBa_I712019(Luciferase Fire fly)-->no colony
+|
-<br>BBa_J332002(LacZ)-->colony
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
-<br>BBa_E2030(Venus YFP)-->not so good
+<tr>
-<br>YFP(P-GEX)-->colony
+<td width="257">Sample DNA</td>
-<br>BBa_F2620-->colony
+<td>3</td>
-<br>BBa_T9002-->colony
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>2</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>7</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>12μL</td>
+</tr>
+</table>
+:From left;
+:(Single Digestion 1~4)
+::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
+::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
+::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
+::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
+:(Double Digestion 1~4)
+::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
+::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
+::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
+::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
+|}
+===21 August, 2008===
+:(20/8)--->'''[[Team:Chiba/protocol/PCR|PCR]]'''
+::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
+::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
+::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
+::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
+::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
-==21,August 2008==
+<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">DNA Template</td>
+<td>1</td>
+</tr>
+<tr>
+<td>dNTP mix</td>
+<td>10</td>
+</tr>
+<tr>
+<td>Foward Primer</td>
+<td>5</td>
+</tr>
+<tr>
+<td>Reverse Primer</td>
+<td>5</td>
+</tr>
+<tr>
+<td>DNA polymerase TAQ</td>
+<td>1</td>
+</tr>
+<tr>
+<td>Thermopol Buffer</td>
+<td>5</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>28</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>50μL</td>
+</tr>
+</table>
-<br>TRANSFORMATION
-<br>BBa_J32007(Lux CDABE)-->no colony
-<br>BBa_B0034(RBS)-->colony
+:95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
-== 22,August 2008 ==
-<br>MINI PREP
-<br>BBa_J52008(Luciferase)
-<br>BBa_F2620
-<br>BBa_T9002
-<br>BBa_J33202(LacZ)
-<br>PUC19
-<br>PGEX VENUS YFP(GST fusion)
-<br>
-<br>TRANSFORMATION
-<br>PGEX VENUS YFP(GST fusion)
+:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+{|align="justify"
+|[[Image:Chiba-0821.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>3</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>2</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>7</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>12μL</td>
+</tr>
+</table>
+:From left;
+::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
+::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
+::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
+::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
+::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK
+|}
+:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
+:competent cells : XL10G
+::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
+::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
+::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
+::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
+::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
+::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
+--->(23/8)'''[[Team:Chiba/protocol/digestion|Digestion]]'''
+===22 August, 2008===
+:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
+:competent cells : XL10G
+::*BBa_J13002(2007) [http://partsregistry.org/Part:BBa_J13002]
+::*BBa_J13002(2006) [http://partsregistry.org/Part:BBa_J13002]
+::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
+::*BBa_J04500(2006) [http://partsregistry.org/Part:BBa_J04500]
+:(Locate & 2 Punch ~iyama ver.~)
+::*BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
+::*BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
+::*BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
+::*BBa_J04500(2008) [http://partsregistry.org/Part:BBa_J04500] -> No colonies on plates
+--->(24/8)'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
+===23 August, 2008===
+:(21/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
+::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
+::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
+::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
+::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
+::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
+::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
+:--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
+::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
+::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
+::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
+::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
+::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
+::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
+::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
+:<table width="500" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="270">Sample No</td>
+<td> 1</td><td> 2</td><td> 3</td><td> 4</td><td> 5</td><td> 6</td><td> 7</td><td> 8</td><td> 9</td><td>10</td><td>11</td><td>12</td><td>13</td><td>14</td><td>15</td><td>16</td><td>17</td><td>18</td><td>19</td><td>20</td><td>21</td>
+</tr><tr>
+<td>BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]</td>
+<td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
+</tr>
+<tr>
+<td>BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]</td>
+<td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
+</tr>
+<tr>
+<td>BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]</td>
+<td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td>
+</tr>
+<tr>
+<td>BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]</td>
+<td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td>
+</tr>
+<tr>
+<tr>
+<td>BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td></tr>
+<tr>
+<td>BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td></tr>
+<tr>
+<td>BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td></tr>
+<tr>
+<td>EcoRⅠ</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
+</tr>
+<tr>
+<td>PstⅠ</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
+</tr>
+<tr>
+<td>Buffer EcoRⅠ</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td>
+</tr>
+<tr>
+<td>BSA</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>3</td><td>3</td><td>3</td><td>3</td><td>3</td><td>3</td><td>1</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+</tr>
+</table>
+:--->[[Team:Chiba/protocol/gelcheck|Gel Check]]
+{|align="justify"
+|[[Image:Chiba-0823-1.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample No</td>
+<td>1~6</td><td>7</td>
+</tr>
+<tr>
+<td>Sample DNA</td>
+<td>3</td><td>1</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>1</td><td>1</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>2</td><td>4</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>6</td><td>6</td>
+</tr>
+</table>
+:From left; Sanple No. 1~7
+::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
+::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
+::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
+::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
+::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
+::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
+::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
+|}
+{|align="justify"
+|[[Image:Chiba-0823-2.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample No</td>
+<td>8~21</td>
+</tr>
+<tr>
+<td>Sample DNA</td>
+<td>10</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>2</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>12</td>
+</tr>
+</table>
+:From left;
+:(Single Digestion) Sanple No. 8~14
+::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
+::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
+::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
+::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
+::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
+::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
+::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
+:(Double Digestion) Sample No. 15~21
+::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
+::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
+::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
+::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
+::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
+::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
+::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK?
+|}
+:'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
+::BBa_J04500(2007:old-sample)[http://partsregistry.org/Part:BBa_J04500]
+<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample No</td>
+<td>1</td><td>2</td><td>3</td>
+</tr>
+<tr>
+<td>Sample DNA</td>
+<td>3</td><td>3</td><td>3</td>
+</tr>
+<tr>
+<td>EcoRⅠ</td>
+<td>-</td><td>0.1</td><td>0.1</td>
+</tr>
+<tr>
+<td>SpeⅠ</td>
+<td>-</td><td>-</td><td>0.1</td>
+</tr>
+<tr>
+<td>EcoRⅠ Buffer</td>
+<td>-</td><td>0.9</td><td>0.8</td>
+</tr>
+<tr>
+<td>BSA</td>
+<td>-</td><td>1</td><td>1</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>-</td><td>5</td><td>5</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>3</td><td>10</td><td>10</td>
+</tr>
+</table>
+--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+{|align="justify"
+|[[Image:Chiba-0823-3.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample No</td>
+<td>1</td><td>2</td><td>3</td>
+</tr>
+<tr>
+<td>Sample DNA</td>
+<td>3</td><td>10</td><td>10</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>1</td><td>2</td><td>2</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>2</td><td>-</td><td>-</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>6</td><td>12</td><td>12</td>
+</tr>
+</table>
+:From left;
+::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
+::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion -> OK
+::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad
+|}
-===[[Team:Chiba/jk/γ/weak 2|>next week]]===
+===[[Team:Chiba/jk/β/weak 2|>next week]]===
 {| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"

Team:Chiba/jk/β/weak 1

From 2008.igem.org

Revision as of 08:46, 25 September 2008

Contents

Week 1

17 August, 2008

18 August, 2008

19 August, 2008

20 August, 2008

21 August, 2008

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