Team:Chiba/jk/γ/week 4

From 2008.igem.org

(Difference between revisions)
(→10,September,2008)
Line 6: Line 6:
== 7,September,2008 ==
== 7,September,2008 ==
-
<br>Cloning
+
*Cloning
-
<br>Digestion
+
[[Team:Chiba/protocol/digestion|Digestion]]
-
<br>vector:BBa_R0040(ptet)① 75ng/μl
+
*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
-
<br>insert:BBa_S03158(RBS+RhlR)② 33.6ng/μl  
+
*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl  
-
<br>insert:BBa_S03156(RBS+lasR)③ 33.6ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
-
<br>insert:BBa_S03160(RBS+cinR)④ 38.4ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
-
<br>vector:BBa_R0010(pLac)⑤ 100ng/μl
+
*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
-
<br>insert:BBa_J52008(RBS+rLuc)⑥ 33.6ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
 +
 
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td>
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td>
</tr>
</tr>
Line 48: Line 49:
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O</td>
<td>1.9</td><td>1</td><td>1</td><td>2</td><td>0.5</td><td>1</td>
<td>1.9</td><td>1</td><td>1</td><td>2</td><td>0.5</td><td>1</td>
</tr>
</tr>
Line 56: Line 57:
</tr>
</tr>
</table>
</table>
-
<br>----->37℃ 3hour
+
 
-
<br>----->Gel Extraction
+
-->37℃ 3hour
 +
 
 +
-->[[Team:Chiba/protocol/ligation/gelex|Gel Extraction]]
 +
 
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td>
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(digestion sample)</td>
+
<td>DNA(digested sample)</td>
<td>6</td><td>10</td><td>10</td><td>10</td><td>4</td><td>10</td>
<td>6</td><td>10</td><td>10</td><td>10</td><td>4</td><td>10</td>
</tr>
</tr>
Line 72: Line 76:
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O</td>
<td>4</td><td>-</td><td>-</td><td>-</td><td>1</td><td>2</td>
<td>4</td><td>-</td><td>-</td><td>-</td><td>1</td><td>2</td>
</tr>
</tr>
Line 83: Line 87:
<td>10</td><td>7</td><td>7</td><td>6</td><td>5</td><td>7</td>
<td>10</td><td>7</td><td>7</td><td>6</td><td>5</td><td>7</td>
</table>
</table>
-
<br>->DNA purification->①-④,⑥->7μl ⑤->6μl
+
 
 +
-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]] (①-④,⑥:7μl ⑤:6μl)
== 8,Septmeber,2008 ==
== 8,Septmeber,2008 ==
-
<br>SAP
+
[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
-
<br>BBa_R0040(ptet)①
+
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)①
-
<br>BBa_R0010(pLac)⑤
+
*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤
 +
 
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>①</td><td>⑤</td>
<td>①</td><td>⑤</td>
</tr>
</tr>
Line 117: Line 123:
</tr>
</tr>
</table>
</table>
-
<br>->37℃ 1hour
+
 
-
<br>->65℃ 30min
+
-->37℃ 1hour
-
<br>->DNA purification
+
 
-
<br>->21μl  6μl
+
-->65℃ 30min
-
<br>->Gel cheack
+
 
 +
-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]]
 +
 
 +
-->21μl  6μl
 +
 
 +
-->[[Team:Chiba/protocol/gelcheck|Gel check]]
 +
 
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>each</td>
<td>each</td>
</tr>
</tr>
Line 136: Line 148:
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
Line 144: Line 156:
</tr>
</tr>
</table>
</table>
-
<br>vector:BBa_R0040(ptet)① 75ng/μl
+
 
-
<br>insert:BBa_S03158(RBS+RhlR)② 33.6ng/μl  
+
*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
-
<br>insert:BBa_S03156(RBS+lasR)③ 33.6ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl  
-
<br>insert:BBa_S03160(RBS+cinR)④ 38.4ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
-
<br>vector:BBa_R0010(pLac)⑤ 100ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
-
<br>insert:BBa_J52008(RBS+rLuc)⑥ 33.6ng/μl
+
*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
 +
*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](RBS+rLuc)⑥ 33.6ng/μl
 +
 
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>①&②</td><td>①&③</td><td>①&④</td><td>①</td><td>⑤&⑥</td><td>⑤</td>
<td>①&②</td><td>①&③</td><td>①&④</td><td>①</td><td>⑤&⑥</td><td>⑤</td>
</tr>
</tr>
Line 172: Line 186:
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O</td>
<td>1</td><td>1</td><td>1</td><td>4</td><td>8.9</td><td>4</td>
<td>1</td><td>1</td><td>1</td><td>4</td><td>8.9</td><td>4</td>
</tr>
</tr>
 +
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
<td>10</td><td>10</td><td>10</td><td>10</td><td>20</td><td>10</td>
<td>10</td><td>10</td><td>10</td><td>10</td><td>20</td><td>10</td>
Line 187: Line 202:
== 10,September,2008 ==
== 10,September,2008 ==
-
<br>[[Team:Chiba/jk/γ/Trl|TIME Responce(liquid)]]
+
[[Team:Chiba/jk/γ/Trl|TIME Responce(liquid)]]
-
<br>[[Team:Chiba/jk/γ/trs|TIME Responce(solid)]]
+
 
-
<br>Colony PCR
+
[[Team:Chiba/jk/γ/trs|TIME Responce(solid)]]
-
<br>->Gel cheack
+
 
-
<br>MINI Prep
+
[[Team:Chiba/protocol/PCR|Colony PCR]]
 +
 
 +
-->[[Team:Chiba/protocol/gelcheck|Gel check]]
 +
 
 +
-->[[Team:Chiba/protocol/DNA_Purification/sigma|Mini prep]]
== 11,September,2008 ==
== 11,September,2008 ==

Revision as of 06:51, 24 October 2008

実験ログ
出力班

Contents

Week 4

7,September,2008

  • Cloning

Digestion

  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
Sample No. ①②③④⑤⑥
DNA(digestion sample) 505454451454
SpeⅠ 100010
PstⅠ 0.50.50.50.50.50.5
XbaⅠ 00.50.50.500.5
BSA(x10) 0.677627
Buffer2 6---2-
Buffer3 -776-7
dH2O 1.91120.51
TOTAL 607070602070

-->37℃ 3hour

-->Gel Extraction

Sample No. ①②③④⑤⑥
DNA(digested sample) 6101010410
Loading Dye 222212
dH2O 4---12
TOTAL 12121212612
well 1077657

-->DNA purification (①-④,⑥:7μl ⑤:6μl)


8,Septmeber,2008

SAP

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)①
  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤
Sample No. ①⑤
DNA(digestion sample) 76
SAP/td> 22
SAP Buffer 33
TOTAL 3030
TOTAL 3030

-->37℃ 1hour

-->65℃ 30min

-->DNA purification

-->21μl 6μl

-->Gel check

Sample No. each
DNA(digestion sample) 1
Loading Dye 1
dH2O 4
TOTAL 6
  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](RBS+rLuc)⑥ 33.6ng/μl
Sample No. ①&②①&③①&④①⑤&⑥⑤
DNA(vector) 333352.1
DNA(insert) 333-3.1-
Ligase 111111
Ligase Buffer(x5) 222222
dH2O 11148.94
TOTAL 101010102010


9,September,2008

10,September,2008

TIME Responce(liquid)

TIME Responce(solid)

Colony PCR

-->Gel check

-->Mini prep

11,September,2008

12,September,2008

13,September,2008


>Last Week
>Next Week



ホーム メンバー紹介 プロジェクト紹介 Parts Submitted to the Registry モデリング ノート
Retrieved from "http://2008.igem.org/Team:Chiba/jk/%CE%B3/week_4"