Team:LCG-UNAM-Mexico/Parts
From 2008.igem.org
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- | <table border="0" cellspacing="0" cellpadding="2" width="590"><tr> | + | <table border="0" cellspacing="0" cellpadding="2" width="590"> |
- | <td class="pageName"><strong>Parts</strong></td> | + | <tr> |
+ | <td colspan="4" class="pageName"><strong>Parts</strong></td> | ||
</tr> | </tr> | ||
- | + | <tr> | |
- | <td | + | <td colspan="4" class="pageName">Constructed Parts</td> |
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | + | <td colspan="4" class="bodyText">The following are the bioparts that we are bringing into play for our project:</td> | |
- | + | </tr> | |
- | + | <tr> | |
+ | <td colspan="3" class="calHeader"> </td> | ||
+ | <td class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="3" class="calHeader">Regulatory Parts</td> | ||
+ | <td width="337" class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png" align="left | ||
+ | "></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">BioBrick Number:</td> | ||
+ | <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119000">BBa_K119000</a></h1></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Part Name:</td> | ||
+ | <td colspan="2" class="bodyText"><strong>Constitutive weak promoter of lacZ</strong></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Description:</td> | ||
+ | <td colspan="2" class="bodyText">LacZ constitutive promoter, without including operators. </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Lenght:</td> | ||
+ | <td colspan="2" class="bodyText">38 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Designed by:</td> | ||
+ | <td colspan="2" class="bodyText">Miguel Angel Ramirez-Romero</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">References:</td> | ||
+ | <td colspan="2" class="bodyText"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader"> </td> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">BioBrick Number:</td> | ||
+ | <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119001">BBa_K119001</a></h1></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Part Name:</td> | ||
+ | <td colspan="2" class="bodyText"><strong>Mutated LacZ promoter</strong></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" >Description:</td> | ||
+ | <td colspan="2" rowspan="2" class="bodyText">Constitutive weak promoter of lacZ which sequence was changed at position 29 (T->G) in order to make it weaker</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" align="up"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Lenght:</td> | ||
+ | <td colspan="2" class="bodyText">38 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Designed by:</td> | ||
+ | <td colspan="2" class="bodyText">Miguel Angel Ramirez-Romero</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">References:</td> | ||
+ | <td colspan="2" class="bodyText"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader"> </td> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">BioBrick Number:</td> | ||
+ | <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119002">BBa_K119002</a></h1></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Part Name:</td> | ||
+ | <td colspan="2" class="bodyText"><strong>RcnR operator (represses RcnA)</strong></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" >Description:</td> | ||
+ | <td colspan="2" class="bodyText">Operator for RcnR(YohL) the negative regulator of niquel extrusion pump(RcnA) in <i>E.coli</i> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Lenght:</td> | ||
+ | <td colspan="2" class="bodyText">83 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Designed by:</td> | ||
+ | <td colspan="2" class="bodyText">Libertad Pantoja</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">References:</td> | ||
+ | <td colspan="2" class="bodyText"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader"> </td> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="3" class="calHeader">Coding Parts</td> | ||
+ | <td class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_coding.png" alt="" align="left | ||
+ | "></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">BioBrick Number:</td> | ||
+ | <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119003">BBa_K119003</a></h1></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Part Name:</td> | ||
+ | <td colspan="2" class="bodyText"><strong>RcnA (YohM)</strong></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" >Description:</td> | ||
+ | <td colspan="2" class="bodyText">Niquel extrusion pump from <i>E.coli</i></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Lenght:</td> | ||
+ | <td colspan="2" class="bodyText">833 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Designed by:</td> | ||
+ | <td colspan="2" rowspan="2" class="bodyText">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">References:</td> | ||
+ | <td colspan="2" class="bodyText"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader"> </td> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="3" class="calHeader">Primers</td> | ||
+ | <td class="calHeader"><img src="http://partsregistry.org/images/partbypart/icon_primer.png" alt="" align="left | ||
+ | "></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">BioBrick Number:</td> | ||
+ | <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119004">BBa_K119004</a></h1></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Part Name:</td> | ||
+ | <td colspan="2" class="bodyText"><strong>Upper primer for aiiA with normal lacZ promoter </strong></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" >Description:</td> | ||
+ | <td colspan="2" rowspan="2" class="bodyText"><p>Upper primer used to add lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter </p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" > </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Lenght:</td> | ||
+ | <td colspan="2" class="bodyText">67 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Designed by:</td> | ||
+ | <td colspan="2" class="bodyText">Martin del Castillo V.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">References:</td> | ||
+ | <td colspan="2" class="bodyText"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader"> </td> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">BioBrick Number:</td> | ||
+ | <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119005">BBa_K119005</a></h1></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Part Name:</td> | ||
+ | <td colspan="2" class="bodyText"><strong>Upper primer for aiiA with mutated lacZ promoter </strong></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" >Description:</td> | ||
+ | <td colspan="2" rowspan="2" class="bodyText"><p>Upper primer used to add a mutated version of lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter </p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" > </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Lenght:</td> | ||
+ | <td colspan="2" class="bodyText">67 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Designed by:</td> | ||
+ | <td colspan="2" class="bodyText">Martin del Castillo V.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">References:</td> | ||
+ | <td colspan="2" class="bodyText"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader"> </td> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">BioBrick Number:</td> | ||
+ | <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119007">BBa_K119007</a></h1></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Part Name:</td> | ||
+ | <td colspan="2" class="bodyText"><strong>Upper primer for RcnA </strong></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" >Description:</td> | ||
+ | <td colspan="2" rowspan="2" class="bodyText"><p>Upper primer used to add cI promoter upstream of the RcnR operator in the device cI+AVGreg_RcnRop_RcnA() </p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" > </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Lenght:</td> | ||
+ | <td colspan="2" class="bodyText">73 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Designed by:</td> | ||
+ | <td colspan="2" class="bodyText">Libertad Pantoja</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">References:</td> | ||
+ | <td colspan="2" class="bodyText"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader"> </td> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">BioBrick Number:</td> | ||
+ | <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119008">BBa_K119008</a></h1></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Part Name:</td> | ||
+ | <td colspan="2" class="bodyText"><strong>Lower primer for RcnA (BBa_K119003) </strong></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" >Description:</td> | ||
+ | <td colspan="2" class="bodyText"><p>Lower primer for RcnA (BBa_K119003). Includes just the end of the RcnA coding region.</p></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Lenght:</td> | ||
+ | <td colspan="2" class="bodyText">21 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Designed by:</td> | ||
+ | <td colspan="2" class="bodyText">Libertad Pantoja</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">References:</td> | ||
+ | <td colspan="2" class="bodyText"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader"> </td> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="3" class="calHeader">Devices</td> | ||
+ | <td class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">BioBrick Number:</td> | ||
+ | <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119009">BBa_K119009</a></h1></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Part Name:</td> | ||
+ | <td colspan="2" class="bodyText"><strong>cI promoter RcnR op RcnA </strong></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" >Description:</td> | ||
+ | <td colspan="2" rowspan="2" class="bodyText"><p>This device encodes the extrusion pump and uses two repressible promoters in order to stop the niquel extrusion. </p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" > </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Lenght:</td> | ||
+ | <td colspan="2" class="bodyText">969 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Designed by:</td> | ||
+ | <td colspan="2" rowspan="2" class="bodyText">Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">References:</td> | ||
+ | <td colspan="2" class="bodyText"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader"> </td> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">BioBrick Number:</td> | ||
+ | <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119010">BBa_K119010</a></h1></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Part Name:</td> | ||
+ | <td colspan="2" class="bodyText"><strong>LuxR_cI_aiiA_Normal lacZ promoter </strong></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" >Description:</td> | ||
+ | <td colspan="2" rowspan="2" class="bodyText"><p>This device encodes cI, the protein which controls RcnA. It also contains LuxR, which Induces the expression of cI and aiiA </p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" > </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Lenght:</td> | ||
+ | <td colspan="2" class="bodyText">2873 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Designed by:</td> | ||
+ | <td colspan="2" class="bodyText">Libertad Pantoja</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">References:</td> | ||
+ | <td colspan="2" class="bodyText"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader"> </td> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">BioBrick Number:</td> | ||
+ | <td colspan="2" class="calHeader"><h1 class="navText"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K119011">BBa_K119011</a></h1></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Part Name:</td> | ||
+ | <td colspan="2" class="bodyText"><strong>LuxR_cI_aiiA_Mutated lacZ promoter </strong></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" >Description:</td> | ||
+ | <td colspan="2" rowspan="2" class="bodyText"><p>This device encodes cI, the protein which controls RcnA. It also contains LuxR, which Induces the expression of cI and aiiA </p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader" > </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Lenght:</td> | ||
+ | <td colspan="2" class="bodyText">2873 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">Designed by:</td> | ||
+ | <td colspan="2" class="bodyText">Libertad Pantoja</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="subHeader">References:</td> | ||
+ | <td colspan="2" class="bodyText"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="108" class="calHeader"> </td> | ||
+ | <td width="33" class="calHeader"> </td> | ||
+ | <td colspan="2" class="calHeader"> </td> | ||
+ | </tr> </table> | ||
+ | <br /> | ||
<br /> | <br /> | ||
<td width="132"> </td> | <td width="132"> </td> |
Revision as of 12:23, 2 October 2008
LCG-UNAM-Mexico | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
iGEM 2008 TEAM | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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