Team:Freiburg/Project

From 2008.igem.org

(Difference between revisions)

Revision as of 23:04, 9 October 2008

Project Report

_projects

under construction

Modular Synthetic Receptor System

Schematical overview of the system

Abstract:
This year´s main project is the attempt to create an "artificial receptor-system", featuring extra- and intracellular modules as well as suitable transmembrane regions. The intracellular domaine of our receptor-device is build by halves of split reporter-proteins that can reassemble and will then produce readable output, e. g. fluorescence. Each one of these protein-halves is connected to its extracellular domaine by a single-span transmembrane-helix. The extracellular or detecting domaine consists of a protein or peptide with the ability to bind a certain molecule.
Now, if a system with two matching receptors is presented these molecules in a strict, pairwise spatial arrangement, the receptor-devices are brought together, the split reporter-protein reassembles inside the cell and the output can be detected. We employ so-called "Origami-DNA" to create the exactly defined molecule-patterns that are needed to activate our receptors. Project Report

@@ Line 14: / Line 14: @@
 Each one of these protein-halves is connected to its extracellular domaine by a single-span transmembrane-helix.
 The extracellular or detecting domaine consists of a protein or peptide with the ability to bind a certain molecule.<br>
-Now, if a system with two matching receptors is presented these molecules in a strict, pairwise spacial arrangement, the receptor-devices are brought together,
+Now, if a system with two matching receptors is presented these molecules in a strict, pairwise spatial arrangement, the receptor-devices are brought together,
 the split reporter-protein reassembles inside the cell and the output can be detected.
 We employ so-called "Origami-DNA" to create the exactly defined molecule-patterns that are needed to activate our receptors.