From 2008.igem.org
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| - | Dear iGEM judges,
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| - | The team LCG-UNAM-MEXICO is participating this year for the first
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| - | time, and I'm writing on behalf of the team to request permission
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| - | for submitting our Biobricks in non-standard vectors.
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| - | Our project requires very tight regulation of E. coli nickel
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| - | extrusion pump (RcnA) through several regulatory upstream proteins.
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| - | Our Analysis suggest that we need to control very accurately the
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| - | production of several molecules and a crucial part of this requires
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| - | stable low copy-number vectors. For this reason we would like to use
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| - | pRK415 and pBBIMCS-5 derivatives as our plasmid backbones. We will
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| - | be adding Biobrick prefixx and suffix to both of them. We will also
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| - | make sure that our parts are easily extracted from the plasmid
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| - | either through digestion with commercially available enzymes or
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| - | through PCR.
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| - | All this information will be shared to ensure that our Biobricks are
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| - | usefull for the community.
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| - | Thanks for your time and consideration
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| - | Sur Herrera Paredes
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| - | LCG-UNAM-MEXICO
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| - | Licenciatura en Ciencias Genómicas
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| - | Universidad Nacional Autónoma de México
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Revision as of 15:31, 30 September 2008
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