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Judging/Variance/Brown
From 2008.igem.org
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Dear iGEM Judging Corps, | Dear iGEM Judging Corps, | ||
| - | + | Here at Brown, we are building a gene network in yeast. We are | |
| - | constructing our parts using the Silver Lab biofusion standard, but | + | constructing our parts using the Silver Lab biofusion standard, but |
| - | in many cases have been doing the final ligation step as a double | + | in many cases have been doing the final ligation step as a double |
| - | insert onto a yeast integration vector from the pRS family. | + | insert onto a yeast integration vector from the pRS family. (As |
| - | (As detailed at the bottom of | + | detailed at the bottom of of http://openwetware.org/wiki/Silver:_BB_Strategy) |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | Thus, our basic parts and intermediates are on biobrick standard | |
| - | + | vectors, but our final constructs are not. The pRS vectors have | |
| + | biobrick sites present, and lack the full prefix or suffix, | ||
| + | depending on the vector. Our parts on pRS vectors can be used as-is | ||
| + | for yeast integration, switched onto biobrick vectors in one | ||
| + | ligation step, or in some cases used as inserts in biobrick | ||
| + | construction. Would you like us to add these parts to the registry | ||
| + | though they are not suitable as vectors for biobrick assembly? | ||
| - | + | Thank you, | |
| - | + | John Szymanski | |
| + | |||
| + | Brown iGEM | ||
| + | Team Limiter | ||
Revision as of 16:23, 30 September 2008
Dear iGEM Judging Corps,
Here at Brown, we are building a gene network in yeast. We are constructing our parts using the Silver Lab biofusion standard, but in many cases have been doing the final ligation step as a double insert onto a yeast integration vector from the pRS family. (As detailed at the bottom of of http://openwetware.org/wiki/Silver:_BB_Strategy)
Thus, our basic parts and intermediates are on biobrick standard vectors, but our final constructs are not. The pRS vectors have biobrick sites present, and lack the full prefix or suffix, depending on the vector. Our parts on pRS vectors can be used as-is for yeast integration, switched onto biobrick vectors in one ligation step, or in some cases used as inserts in biobrick construction. Would you like us to add these parts to the registry though they are not suitable as vectors for biobrick assembly?
Thank you, John Szymanski
Brown iGEM Team Limiter
