Alberta NINT/9 June 2008

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(Difference between revisions)
(New page: lab work (SD): K102008 and K102008 negative control plates again had >100 colonies each. Verified sequencing for K102002. Digested K102002 with Ba...)
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             Digested K102002 with BamHI, with NcoI, and with BamHI+NcoI to test the digestion.
             Digested K102002 with BamHI, with NcoI, and with BamHI+NcoI to test the digestion.
             Ran DNA from digest on 2% agarose gel.
             Ran DNA from digest on 2% agarose gel.
 +
            Gel reveals that digest of K102002 with BamHI formed two fragments.  Conclusion: K102002 contains
 +
            two BamHI restriction sites.  A closer examination of the sequences indicates BBa_E0433 (LacZ alpha)
 +
            contains a BamHI restriction site.  A new version of LacZ alpha is required.
 +
            BBa_I732018 is LacZ alpha but lacks the BamHI restriction site.  The DNA for this part is isolated
 +
            from the iGEM BioBricks parts registry book according to the standard procedure.

Revision as of 22:25, 9 June 2008

lab work (SD): 

            K102008 and K102008 negative control plates again had >100 colonies each.  
            Verified sequencing for K102002.
            Digested K102002 with BamHI, with NcoI, and with BamHI+NcoI to test the digestion.
            Ran DNA from digest on 2% agarose gel.
            Gel reveals that digest of K102002 with BamHI formed two fragments.  Conclusion: K102002 contains
            two BamHI restriction sites.  A closer examination of the sequences indicates BBa_E0433 (LacZ alpha)
            contains a BamHI restriction site.  A new version of LacZ alpha is required.
            BBa_I732018 is LacZ alpha but lacks the BamHI restriction site.  The DNA for this part is isolated 
            from the iGEM BioBricks parts registry book according to the standard procedure.
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