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Alberta NINT/9 June 2008
From 2008.igem.org
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two BamHI restriction sites. A closer examination of the sequences indicates BBa_E0433 (LacZ alpha) | two BamHI restriction sites. A closer examination of the sequences indicates BBa_E0433 (LacZ alpha) | ||
contains a BamHI restriction site. A new version of LacZ alpha is required. | contains a BamHI restriction site. A new version of LacZ alpha is required. | ||
| + | <br><br> | ||
BBa_I732018 is LacZ alpha but lacks the BamHI restriction site. The DNA for this part is isolated | BBa_I732018 is LacZ alpha but lacks the BamHI restriction site. The DNA for this part is isolated | ||
from the iGEM BioBricks parts registry book according to the standard procedure. | from the iGEM BioBricks parts registry book according to the standard procedure. | ||
Revision as of 22:25, 9 June 2008
lab work (SD):
K102008 and K102008 negative control plates again had >100 colonies each.
Verified sequencing for K102002.
Digested K102002 with BamHI, with NcoI, and with BamHI+NcoI to test the digestion.
Ran DNA from digest on 2% agarose gel.
Gel reveals that digest of K102002 with BamHI formed two fragments. Conclusion: K102002 contains
two BamHI restriction sites. A closer examination of the sequences indicates BBa_E0433 (LacZ alpha)
contains a BamHI restriction site. A new version of LacZ alpha is required.
BBa_I732018 is LacZ alpha but lacks the BamHI restriction site. The DNA for this part is isolated
from the iGEM BioBricks parts registry book according to the standard procedure.
