Team:Warsaw/Calendar-Main/16 July 2008

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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a>  strain with ligation from previous day </li>
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a>  strain with ligation from previous day </li>
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Revision as of 21:39, 1 October 2008

Gallery Bricks Notebook Team Project Home


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Cloning of protein Z DNA to OmpA constructs
Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha).
  2. Control digest of isolated plasmids with BamHI and SacI (we found 4 good clones).


Cloning of protein A DNA to geneart plasmid in place of protein Z DNA
Antoni

  1. Transformation of E. coli TOP10 strain with ligation from previous day

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