Team:Warsaw/Calendar-Main/16 July 2008

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<li> Control digest of isolated plasmids with BamHI and SacI (we found 4 good clones).</li></ol></p>
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<h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>Antoni</h3>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a>  strain with ligation from previous day </li>
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Revision as of 23:36, 2 October 2008

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Cloning of protein Z DNA to OmpA constructs
Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha).
  2. Control digest of isolated plasmids with BamHI and SacI (we found 4 good clones).

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