Team:Warsaw/Calendar-Main/24 July 2008

(Difference between revisions)

Revision as of 23:29, 2 October 2008


	Attribution analyzer / Interactive list of topics

1. Separate transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin.

1. Preparation of chemocompetent bacteria E. coli TOP10.

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 . Preparation of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> TOP10.<br>
-<br>
-<h3>Cloning of protein A DNA to pET15b+Omp-alfa plasmid<br>Antoni</h3>
-<p><ol>
-<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI, pET15b+OmpA_alpha<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> dephosphorylation with CIAP</a> </li> </li>
-<li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands  </li>
-<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of pET15b+alfa and A </li>
-<li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a>. </li>
-<li> Transformants plating on LB + ampitilin.</li>
-</ol></p>