Team:Warsaw/Calendar-Main/17 June 2008

(Difference between revisions)

Revision as of 11:02, 6 October 2008


	Attribution analyzer / Interactive list of topics

PCR on OmpA_linker and linker_alpha with OmpaL_N and AlphaP_XB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
PCR on OmpA_linker and linker_omega with OmpaL_N and OmegaP_EPB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
Gel electrophoresis of PCR products and gel-out of OmpA_alpha and OmpA_omega.
Electrophoresis to estimate the concentration of isolated DNA.
Overnight digest of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI.
Inoculation of E. coli TOP10 with pACYC177 plasmid into liquid LB + kanamycin.

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 <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of OmpA_alpha and OmpA_omega. </li>
 <li>Electrophoresis to estimate the concentration of isolated DNA.</li>
-<li> Overnight <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI. </li></ol>
+<li> Overnight <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI. </li>
-<li> Inoculation of  <i>E. coli</i> TOP10 with pACYC177 plasmid into liquid LB + kanamycin. </li>
+<li> Inoculation of  <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with pACYC177 plasmid into liquid LB + kanamycin. </li>
-</html>
+</ol></html>
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