Team:Warsaw/Calendar-Main/1 July 2008

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<h3>Cloning alpha-A and omega-A fusions on pKS</h3>
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<h4>Michał L., Ewa, Marcin:</h4>
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<ol>
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<li>Isolation of plasmids from transformants</li>
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<li>Control digest of plasmids with SacI+NotI</li>
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<li>Gel electrophoresis of digested DNA</li>
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</ol>
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<p>We have successfully cloned A-alpha and A-omega fusions on pKS vector. The DNA will be now sequenced to ensure that our constructs are correct.</p>
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<h3>Preparation of constructs with OmpA protein fusions<br>Paweł</h3>
<h3>Preparation of constructs with OmpA protein fusions<br>Paweł</h3>

Revision as of 11:48, 11 October 2008

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Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin:

  1. Isolation of plasmids from transformants
  2. Control digest of plasmids with SacI+NotI
  3. Gel electrophoresis of digested DNA

We have successfully cloned A-alpha and A-omega fusions on pKS vector. The DNA will be now sequenced to ensure that our constructs are correct.

Preparation of constructs with OmpA protein fusions
Paweł

  1. Isolation of pET15b plasmid.
  2. Overnight digest of pET15b plasmid with NdeI and BamHI, dephosphorylation with CIAP.

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