Team:Warsaw/Calendar-Main/9 May 2008

(Difference between revisions)

Revision as of 08:48, 11 October 2008


	Attribution analyzer / Interactive list of topics

Restriction digest of pTrc99A-AID with HindIII and NcoI (1x Tango buffer).
DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp).
Restriction digest of pMPM-T5 with HindIII and NcoI (1x Tango buffer), dephosphorylation (CIAP).
DNA electrophoresis and isolation of pMPM-T5 vector from proper band (4250 bp).

1-DNA ladder; 2-pMPM-T5 digested with NcoI and HindIII

1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII

@@ Line 10: / Line 10: @@
 <li> DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA</a> encoding AID from proper band (600 bp).</li>
-<img src="https://static.igem.org/mediawiki/2008/8/89/Gel_out_aid_WAW.jpg" width=300/>
-<var>1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII</var>
 <li> Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with HindIII and NcoI (1x Tango buffer), <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation</a> (CIAP).</li>
@@ Line 20: / Line 19: @@
 <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments. </li>
 </ol></p>
+<img src="https://static.igem.org/mediawiki/2008/8/89/Gel_out_aid_WAW.jpg" width=300/>
+<var>1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII</var>
 <div align="center"><img src="http://openwetware.org/images/e/e1/UW_AID.jpg" height="340"/></div>
 </html>