From 2008.igem.org
(Difference between revisions)
|
|
Line 28: |
Line 28: |
| | | |
| | | |
- | <h3>Cloning of protein A DNA to OmpA constructs</h3>
| |
- | <ol><li>Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega. Primers used:
| |
| | | |
- | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a></li>
| |
- | <li>Confirmed transformant colonies inoculated to liquid LB with kanamycin. </li>
| |
- | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (pACYC177+OmpA_A_alpha). </li>
| |
- | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI.</li></ol>
| |
- | </p>
| |
| | | |
| | | |
Revision as of 20:23, 11 October 2008
![](https://static.igem.org/mediawiki/2008/5/52/Spacer.gif) |
![](https://static.igem.org/mediawiki/2008/5/52/Spacer.gif) |
![](http://2008.igem.org/wiki/images/6/62/Logo_notebook.jpg) |
![](https://static.igem.org/mediawiki/2008/5/52/Spacer.gif) |
Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPaweł
- Results of ligation: 6 colonies grown.
- Each colony cultured overnight in LB + ampicillin.
- Separate transformant colonies (tranformation of ligation of pACYC177+OmpA_alpha and omega_A from previous day) inoculated to liquid LB with kanamycin.
Antoni:
- Preparation of chemocompetent bacteria E. coli TOP10.
Cloning omega-A fusion on pKS (second attempt)
Michał L., Ewa, Marcin:
Sequencing confirms that we have obtained proper construct with omega-A fusion
|
![](https://static.igem.org/mediawiki/2008/5/52/Spacer.gif) |