Team:Warsaw/Calendar-Main/24 July 2008

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<h4>Antoni:</h4>
<h4>Antoni:</h4>
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<ol><li>Preparation of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htmtop10">TOP10</a>.</li></ol>
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<p>Preparation of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htmtop10">TOP10</a>.</p>
<h3>Cloning omega-A fusion on pKS (second attempt)</h3>
<h3>Cloning omega-A fusion on pKS (second attempt)</h3>

Revision as of 20:33, 11 October 2008

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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA

Paweł

  1. Results of ligation: 6 colonies grown.
  2. Each colony cultured overnight in LB + ampicillin.
  1. Separate transformant colonies (tranformation of ligation of pACYC177+OmpA_alpha and omega_A from previous day) inoculated to liquid LB with kanamycin.

Antoni:

Preparation of chemocompetent bacteria E. coli TOP10.

Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin:

Sequencing confirms that we have obtained proper construct with omega-A fusion

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