Team:Warsaw/Calendar-Main/24 July 2008

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(Difference between revisions)
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<li>Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega. Primers used:
<li>Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega. Primers used:
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a></li>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>.</li><li> Gel electrophoresis.</li>
<li>Confirmed transformant colonies inoculated to liquid LB with kanamycin. </li></ol>
<li>Confirmed transformant colonies inoculated to liquid LB with kanamycin. </li></ol>

Revision as of 22:43, 11 October 2008

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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA

Paweł

  1. Results of ligation: 6 colonies grown.
  2. Each colony cultured overnight in LB + ampicillin.

Cloning of protein A DNA to OmpA constructs

Michał K.

  1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega. Primers used: AL+SacI and AP+NotI.
  2. Gel electrophoresis.
  3. Confirmed transformant colonies inoculated to liquid LB with kanamycin.

Antoni

Preparation of chemocompetent bacteria E. coli TOP10.

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