Team:Warsaw/Calendar-Main/9 May 2008

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Revision as of 23:01, 11 October 2008


	Attribution analyzer / Interactive list of topics

Restriction digest of pTrc99A-AID with HindIII and NcoI (1x Tango buffer).
DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp). Fig. 1.
Restriction digest of pMPM-T5 with HindIII and NcoI (1x Tango buffer), dephosphorylation (CIAP).
DNA electrophoresis and isolation of pMPM-T5 vector from proper band (4250 bp). Fig. 2.
Electrophoresis to estimate the concentration of isolated DNA.
Ligation of isolated DNA fragments.

Fig. 1. 1-DNA ladder; 2-pTRC99a-AID digested with NcoI and HindIII

Fig. 2. 1-DNA ladder; 2-pMPM-T5 digested with NcoI and HindIII

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 <li> Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with HindIII and NcoI (1x Tango buffer), <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation</a> (CIAP).</li>
-<li> DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band (4250 bp). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/6_May_2008#fig2">Fig. 2</a>.</li>
+<li> DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band (4250 bp). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/9_May_2008#fig2">Fig. 2</a>.</li>
 <li>Electrophoresis to estimate the concentration of isolated DNA.</li>