Team:Warsaw/Calendar-Main/27 September 2008

From 2008.igem.org

(Difference between revisions)
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<li>ADelL+KpnI and ADelP primers mutagenesis:</li>
<li>ADelL+KpnI and ADelP primers mutagenesis:</li>
<ul>
<ul>
-
<li>pACYClac+ompA-A-omega: <b>0</b> positive, <b>5</b> negative</li>
+
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYClac+ompA-A-omega</a>: <b>0</b> positive, <b>5</b> negative</li>
-
<li>pACYClac+ompa-A-alpha: <b>3</b> positive, <b>7</b> negative</li>
+
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYClac+ompa-A-alpha</a>: <b>3</b> positive, <b>7</b> negative</li>
<li>pACYClac+ ompa-alpha-A: <b>1</b> positive, <b>2</b> negative</li>
<li>pACYClac+ ompa-alpha-A: <b>1</b> positive, <b>2</b> negative</li>
</ul>
</ul>
<li>AMutL+KpnI and AMutP mutagenesis:</li>
<li>AMutL+KpnI and AMutP mutagenesis:</li>
<ul>
<ul>
-
<li>pACYClac+ompA-A-omega: <b>4</b> positive, <b>16</b> negative</li>
+
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYClac+ompA-A-omega</a>: <b>4</b> positive, <b>16</b> negative</li>
-
<li>pACYClac+ompa-A-alpha: <b>4</b> positive, <b>8</b> negative</li>
+
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYClac+ompa-A-alpha</a>: <b>4</b> positive, <b>8</b> negative</li>
<li>pACYClac+ ompa-alpha-A: <b>2</b> positive, <b>15</b> negative</li>
<li>pACYClac+ ompa-alpha-A: <b>2</b> positive, <b>15</b> negative</li>
</ul>
</ul>

Revision as of 21:21, 19 October 2008

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Mutagenesis of protein A

Paweł

  1. Isolation of plasmid DNA from overnight cultures (it was really tough work...).
  2. Control digestion of isolated plasmids with KpnI and BamHI (if mutagenesis really occured, ~550bp band should be visible).
  3. Results of digestion:
  4. Plasmids which gave positive digestion results prepared for sequencing (one sample from each variant which gave positive result).

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