Team:Paris/Modeling/f3bis

(Difference between revisions)

Revision as of 08:39, 16 October 2008

We have [EnvZ]_produced = {coef_env}expr(pTet) = {coef_env} ƒ1([aTc]_i)

and [EnvZ]_total = [EnvZ]_basal + [EnvZ]_produced

and [FliA] = {coef_FliA}expr(pBad) = {coef_FliA} ƒ2([arab]_i)

So, if we denote phosphorylated OmpR by OmpR^*, we have

that we can then introduce in the previous expression (ƒ 3) :

param	signification	unit	value
[expr(pFlhDC)]	expression rate of pFlhDC with RBS E0032	nM.s^-1	see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM?
γ_GFP	dilution-degradation rate of GFP(mut3b)	min^-1	0.0198
[GFP]	GFP concentration at steady-state	nM	need for 20 + 20 measures and 5x5 measures for the SUM?
(fluorescence)	value of the observed fluorescence	au	need for 20 + 20 measures and 5x5 measures for the SUM?
conversion	conversion ratio between fluorescence and concentration	nM.au^-1	(1/79.429)

param	signification corresponding parameters in the equations	unit	value
β_12bis	production rate of FliA-pFlhDC with RBS E0032 replace β₁₂ (not written)	nM.min^-1
(K_11bis/{coef_fliA})	activation constant of FliA-pFlhDC K_11bis	nM
n_11bis	complexation order of FliA-pFlhDC replace n₁₁ (not written)	no dimension
β_2bis	production rate of EnvZ-pFlhDC with RBS E0032 β_2bis	nM.min^-1
(K_19bis/{coef_envZ})	activation constant of EnvZ-pFlhDC replace K₁₉ (not written)	nM
n_19bis	complexation order of EnvZ-pFlhDC n_19bis	no dimension

Then, if we have time, we want to verify the expected relation

Revision as of 08:39, 16 October 2008 (view source) Hugo (Talk \| contribs) ← Older edit		Revision as of 08:39, 16 October 2008 (view source) Hugo (Talk \| contribs) Newer edit →
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-	[[Image:f3env.png\|thumb]] (see [[Team:Paris/Modeling/Oscillations#Biochemical_Assumptions\|the considerations on the use of EnvZ]]	+	[[Image:f3env.png\|thumb]] (see [[Team:Paris/Modeling/Oscillations#Biochemical_Assumptions\|the considerations on the use of EnvZ]])

	We have [EnvZ]<sub>produced</sub> = {coef<sub>env</sub>}''expr(pTet)'' = {coef<sub>env</sub>} 1([aTc]<sub>i</sub>)		We have [EnvZ]<sub>produced</sub> = {coef<sub>env</sub>}''expr(pTet)'' = {coef<sub>env</sub>} 1([aTc]<sub>i</sub>)