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Team:Hawaii/Notebook/2008-06- 5
From 2008.igem.org
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| - | <blockquote>"So Grace, what promoter are going use?"<br> | + | <blockquote>"So Grace, what promoter are you going use?"<br> |
:::"Um... my name is Krystle."</blockquote> | :::"Um... my name is Krystle."</blockquote> | ||
Latest revision as of 03:27, 12 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Contents |
What we did today
Wetlab work
Made PCR gel
- Norman
- 12g Agarose
- 12 ml 50X TAE
- 588 ml nanopure water
- 6 μl EtBr
Transformed DH5α with PRL1383a
- Grace & Krystle
- Used same protocol as competency test on 6/3/08
- Shaking at 235 rpm
- Plated on LB+sp plate
- E. coli from batch 2 (2-14) (OD600=0.3)
Discussion
- GK and KS will read up on PCC6803 transformation tonight; transformation will be conducted tomorrow morning.
- Tomorrow afternoon, we will practice extracting BioBrick plasmids off filter paper.
- Also need to make TE tomorrow.
Quote of the day
"So Grace, what promoter are you going use?"
- "Um... my name is Krystle."
"Okay, see you all next week. Bye Grace... er... Margaret."[http://manoa.hawaii.edu/][http://manoa.hawaii.edu/ovcrge/
][http://www.ctahr.hawaii.edu
]
