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Team:Paris/Modeling/f3bis
From 2008.igem.org
(Difference between revisions)
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| - | | | + | |(K<sub>21</sub><sup>eff</sup>/{coef<sub>fliA</sub>}) |
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|activation constant of FliA-pFlhDC <br> K<sub>12</sub> | |activation constant of FliA-pFlhDC <br> K<sub>12</sub> | ||
|nM | |nM | ||
Revision as of 10:52, 16 October 2008
We have [EnvZ]produced = {coefenv}expr(pTet) = {coefenv} ƒ1([aTc]i)
and [EnvZ]total = [EnvZ]b + [EnvZ]produced
and [FliA] = {coefFliA}expr(pBad) = {coefFliA} ƒ2([arab]i)
So, if we denote phosphorylated OmpR by OmpR*, we have
that we can then introduce in the previous expression (see ƒ3) :
| param | signification | unit | value | comments |
| [expr(pFlhDC)] | expression rate of pFlhDC with RBS E0032 | nM.min-1 | need for 20 mesures with well choosen values of [aTc]i | |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 | |
| [GFP] | GFP concentration at steady-state | nM | need for 20 | |
| (fluorescence) | value of the observed fluorescence | au | need for 20 | |
| conversion | conversion ratio between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value | comments |
| (K21eff/{coeffliA}) | activation constant of FliA-pFlhDC K12 | nM | ||
| n12 | complexation order of FliA-pFlhDC n12 | no dimension | ||
| β2 | production rate of OmpR-pFlhDC with RBS E0032 β2 | nM.min-1 | ||
| (K22/{coefomp}) | activation constant of OmpR-pFlhDC K22 | nM | ||
| n22 | complexation order of OmpR-pFlhDC n22 | no dimension |
