Team:Hawaii/Notebook/2008-10-17

From 2008.igem.org

(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Restreaked BBpRL1383a construct=== :<strong> Grace</strong> :* Looked at J33207+pRL1383a (BBpRL1383a) plates. 50 μl...)
(Restreaked BBpRL1383a construct)
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:* Looked at J33207+pRL1383a (BBpRL1383a) plates. 50 &mu;l plate had blue colonies. Restreaked on LB+sp<sub>2.5</sub>+sm<sub>2.5</sub> + X-gal
:* Looked at J33207+pRL1383a (BBpRL1383a) plates. 50 &mu;l plate had blue colonies. Restreaked on LB+sp<sub>2.5</sub>+sm<sub>2.5</sub> + X-gal
 +
===Inoculated for plasmid prep===
 +
:<strong>Grace</strong>
 +
 +
:* Inoculated 200 ml TB+sp<sub>2.5</sub>+sm<sub>2.5</sub> with blue colony. Incubated over the weekend at 37C with shaking at 250 rpm.
= Discussion =
= Discussion =

Revision as of 00:57, 18 October 2008

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Things we did today

Wetlab work

Restreaked BBpRL1383a construct

Grace
  • Looked at J33207+pRL1383a (BBpRL1383a) plates. 50 μl plate had blue colonies. Restreaked on LB+sp2.5+sm2.5 + X-gal

Inoculated for plasmid prep

Grace
  • Inoculated 200 ml TB+sp2.5+sm2.5 with blue colony. Incubated over the weekend at 37C with shaking at 250 rpm.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]