Experiment
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+ | <td height="59" colspan="3" valign="bottom" bgcolor="#03438A"><span class="STYLE28"> <img src="https://static.igem.org/mediawiki/2008/d/dc/11.JPG" width="61" height="43"> Experiment results </span></td> | ||
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+ | <td height="53" colspan="3" align="center" valign="middle" bgcolor="#03438A"><p><span class="STYLE32">Synthetic convertible ecosystem</span><BR> | ||
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+ | <td height="54" align="center" valign="middle" bgcolor="#03438A"><div class="STYLE38">EG cells received 3OC6HSL signal molecular and expressed ampicillin resistance protein</div></td> | ||
+ | <td> </td> | ||
+ | <td align="center" valign="middle" bgcolor="#03438A"><div class="STYLE39">ER cells received C4HSL signal molecular and expressed kanamycin resistance protein</div></td> | ||
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+ | <td height="366" align="center" valign="middle" bgcolor="#03438A"><img src="https://static.igem.org/mediawiki/2008/thumb/5/57/113.JPG/772px-113.JPG" width="459" height="354"></td> | ||
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+ | <td align="center" valign="middle" bgcolor="#03438A"><img src="https://static.igem.org/mediawiki/2008/thumb/c/c9/112.JPG/772px-112.JPG" width="459" height="355"></td> | ||
+ | </tr> | ||
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+ | <td height="42" align="center" valign="top" bgcolor="#03438A"><div class="STYLE36">The growth curve and the expression of the GFP of the EG cells in different condition</div></td> | ||
+ | <td></td> | ||
+ | <td align="center" valign="top" bgcolor="#03438A"><div class="STYLE36">The growth curve and the expression of the GFP of the ER cells in different condition</div></td> | ||
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+ | <td height="51" colspan="3" valign="middle" bgcolor="#03438A"><div class="STYLE39">ER overwhelms EG in population at the existence of antibiotics </div></td> | ||
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+ | <td height="492" align="center" valign="middle" bgcolor="#03438A"><img src="https://static.igem.org/mediawiki/2008/4/4a/117.JPG" width="392" height="474"></td> | ||
+ | <td> </td> | ||
+ | <td align="center" valign="middle" bgcolor="#03438A"><img src="https://static.igem.org/mediawiki/2008/thumb/d/d5/115.jpg/635px-115.jpg" width="458" height="431"></td> | ||
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+ | <td height="46" colspan="2" valign="middle" bgcolor="#03438A"><div><span class="STYLE39"><strong>Antibiotic changes detected by </strong>LC-MS/MS</span></div></td> | ||
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+ | <td height="422" valign="middle" bgcolor="#03438A"><img src="https://static.igem.org/mediawiki/2008/7/7c/110.jpg" width="520" height="415"></td> | ||
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+ | <td width="418" valign="middle"><div class="STYLE36"><span class="STYLE40">B</span>oth ampicillin and kanamycin decrease with the increase of population density due to gradual decomposition of antibiotics. Most of antibiotics were decomposed at the lag phase and decreased very quickly to a low level at the beginning of the exponential phase. </div></td> | ||
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+ | <td height="41" colspan="5" valign="middle" bgcolor="#03438A"><div class="STYLE39">Increase of initial population density enhances system resistance to antibiotics</div></td> | ||
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+ | <td height="45" valign="top" bgcolor="#03438A"><div> | ||
+ | <div class="STYLE36">EG= 3.33×106/ml </div> | ||
+ | <div class="STYLE36">ER= 1×106/ml</div> | ||
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+ | <div class="STYLE36">EG= 5×106/ml </div> | ||
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+ | <div class="STYLE36">EG= 1×107/ml </div> | ||
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+ | <td height="41" colspan="7" valign="top" bgcolor="#03438A"><p> </p> </td> | ||
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+ | </tr> | ||
+ | <tr> | ||
+ | <td height="25" align="center" valign="top" bgcolor="#03438A"><span class="STYLE36">simulation</span></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </table></td> | ||
+ | <td> </td> | ||
+ | <td colspan="3" valign="top"><table width="100%" border="0" cellpadding="0" cellspacing="0" bgcolor="#03438A"> | ||
+ | <!--DWLayoutTable--> | ||
+ | <tr> | ||
+ | <td width="13" height="422"> </td> | ||
+ | <td width="411" valign="top"><!--DWLayoutEmptyCell--> </td> | ||
+ | </tr> | ||
+ | </table></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td height="6"></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </table></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td height="735"></td> | ||
+ | <td valign="top"><table width="100%" border="0" cellpadding="0" cellspacing="0"> | ||
+ | <!--DWLayoutTable--> | ||
+ | <tr> | ||
+ | <td height="41" colspan="7" valign="middle" bgcolor="#03438A"><div class="STYLE42">Influence of antibiotics on population dynamics</div></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="312" height="6"></td> | ||
+ | <td width="6"></td> | ||
+ | <td width="201"></td> | ||
+ | <td width="8"></td> | ||
+ | <td width="102"></td> | ||
+ | <td width="7"></td> | ||
+ | <td width="313"></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td height="293" valign="top"><table width="100%" border="0" cellpadding="0" cellspacing="0"> | ||
+ | <!--DWLayoutTable--> | ||
+ | <tr> | ||
+ | <td width="312" height="250" align="center" valign="middle" bgcolor="#03438A"><img src="https://static.igem.org/mediawiki/2008/f/fe/31.JPG" width="301" height="236"></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td height="43" valign="top" bgcolor="#03438A"><div class="STYLE36">EG= 1×107/ml</div></td> | ||
+ | </tr> | ||
+ | |||
+ | </table></td> | ||
+ | <td> </td> | ||
+ | <td colspan="3" valign="top"><table width="100%" border="0" cellpadding="0" cellspacing="0"> | ||
+ | <!--DWLayoutTable--> | ||
+ | <tr> | ||
+ | <td width="311" height="250" align="center" valign="middle" bgcolor="#03438A"><img src="https://static.igem.org/mediawiki/2008/8/85/32.jpg" width="302" height="236"></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td height="43" valign="top" bgcolor="#03438A"><div class="STYLE36">ER= 1×106/ml</div></td> | ||
+ | </tr> | ||
+ | |||
+ | </table></td> | ||
+ | <td> </td> | ||
+ | <td valign="top"><table width="100%" border="0" cellpadding="0" cellspacing="0"> | ||
+ | <!--DWLayoutTable--> | ||
+ | <tr> | ||
+ | <td width="313" height="250" align="center" valign="middle" bgcolor="#03438A"><img src="https://static.igem.org/mediawiki/2008/d/da/33.jpg" width="304" height="236"></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td height="43" valign="top" bgcolor="#03438A"><div> | ||
+ | <div> | ||
+ | <div class="STYLE36">EG= 5×106/ml </div> | ||
+ | <div class="STYLE36">ER= 1×106/ml</div> | ||
+ | </div> | ||
+ | </div> </td> | ||
+ | </tr> | ||
+ | |||
+ | </table> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td height="5"></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td height="382" colspan="3" valign="top"><table width="100%" border="0" cellpadding="0" cellspacing="0"> | ||
+ | <!--DWLayoutTable--> | ||
+ | <tr> | ||
+ | <td width="519" height="349" align="center" valign="middle" bgcolor="#03438A"><img src="https://static.igem.org/mediawiki/2008/f/fe/34.jpg" width="457" height="337"></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td height="33" align="center" valign="top" bgcolor="#03438A"><span class="STYLE36">simulation</span></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </table></td> | ||
+ | <td> </td> | ||
+ | <td colspan="3" valign="top"><table width="100%" border="0" cellpadding="0" cellspacing="0" bgcolor="#03438A"> | ||
+ | <!--DWLayoutTable--> | ||
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+ | <td width="412" valign="top"><!--DWLayoutEmptyCell--> </td> | ||
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+ | <td height="8"></td> | ||
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+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
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+ | |||
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+ | </table></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td height="44"></td> | ||
+ | <td> </td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
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+ | </table></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td height=" | + | <td height="36"> </td> |
+ | <td width="459"> </td> | ||
<td> </td> | <td> </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | <br /> | ||
</body> | </body> | ||
</html> | </html> |
Revision as of 10:10, 28 October 2008
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