Team:Hawaii/Notebook/2008-10-23
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Antibiotic test=== :<strong> Grace</strong> :* No change from yesterda...) |
(→Verification of transformants) |
||
Line 10: | Line 10: | ||
===Verification of transformants=== | ===Verification of transformants=== | ||
+ | [[Image:102308colonyPCR.png|right|thumb|500px|EtBr stained 2% agarose gel ran at 95V for 1 hour. Two microliters of PCR product were loaded into each lane.]] | ||
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
{|border=1 align=center | {|border=1 align=center | ||
Line 29: | Line 30: | ||
|} | |} | ||
:* Colony PCR of 4 BB-1 transformants | :* Colony PCR of 4 BB-1 transformants | ||
- | |||
= Discussion = | = Discussion = |
Revision as of 21:45, 23 October 2008
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Antibiotic test
- Grace
- No change from yesterday
- Colony PCR of 6 colonies from sp40, 50, 75 and 8 colonies from sp100 plates
Verification of transformants
- Grace
Plasmid | Colonies |
---|---|
J04430 | 0 |
(-) on amp100 | 0 |
BB-1 | 4 |
(-) on sp100 | 0 |
- Colony PCR of 4 BB-1 transformants
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]