Team:Chiba/Calendar-Home/31 August 2008

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Revision as of 11:38, 24 October 2008

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30 August 2008 <|> 1 September 2008

Laboratory work

Team:Input

no work

Team:Communication

Transformation

Competent Cells : XL10Gold

[http://partsregistry.org/Part:BBa_K084007 BBa_K084007](Plac+RBS+LasI)
[http://partsregistry.org/Part:BBa_K084008 BBa_K084008](Plac+RBS+RhlI)

---> We saved these plates with a refrigerator.

Digestion

[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)

Sample No.	1~3	4
Sample DNA(μL)	12	10
PstⅠ(μL)	0.2	0.2
XbaⅠ(μL)	0.2	-
SpeⅠ(μL)	-	0.4
Buffer 2(μL)	-	1.5
Buffer 3(μL)	2	-
BSA(μL)	2	1.5
dH₂O(μL)	3.6	1.4
TOTAL(μL)	20	15

--->(1/9)Gel Check

(30/8)--->Mini prep

--->Digestion test

[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②

Sample	Single Digesiton 2~5	Double Digestion 2~5	Single Digestion T9002①	Single Digesiton T9002②	Double Digestion T9002①②
Sample DNA(μL)	1	3	3	1	3
XbaⅠ(μL)	0.1	0.1	0.1	0.1	0.1
SpeⅠ(μL)	-	0.1	-	-	0.1
Buffer 2(μL)	0.9	0.8	0.9	0.9	0.8
BSA(μL)	1	1	1	1	1
dH₂O(μL)	7	5	5	7	5
TOTAL(μL)	10	10	10	10	10

--->Gel Check

Sample No.	[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①②	Single & Double Digestion
Sample DNA(μL)	1	10
Loading Dye(μL)	1	2
dH₂O(μL)	4	-
TOTAL(μL)	6	12

From Left

Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②

Team:Output

Digestion

[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
PCR product([http://partsregistry.org/Part:BBa_J52008 BBa_J52008])②

Sample No.	①	②
DNA(μL)	10	10
SpeⅠ(μL)	1	0
PstⅠ(μL)	1	1
XbaⅠ(μL)	0	1
BSA(x100)(μL)	0.5	0.5
Buffer2(μL)	5	0
Buffer3(μL)	0	5
dH₂O(μL)	28.5	28.5
TOTAL(μL)	50	50

-->left for 3 hours at 37 degrees.

-->①20μl refine

-->②20μl refine

@@ Line 9: / Line 9: @@
 ===Team:Communication===
 :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
-:Competent Cells : XL10G
+:Competent Cells : XL10Gold
-::*[http://partsregistry.org/Part:BBa_K084007 BBa_K084007(Plac+RBS+LasI)]
+::*[http://partsregistry.org/Part:BBa_K084007 BBa_K084007](Plac+RBS+LasI)
-::*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008(Plac+RBS+RhlI)]
+::*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008](Plac+RBS+RhlI)
 ::---> We saved these plates with a refrigerator.
@@ Line 28: / Line 28: @@
 </tr>
 <tr>
-<td>Sample DNA</td><td>12</td><td>10</td>
+<td>Sample DNA(μL)</td><td>12</td><td>10</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstⅠ(μL)</td>
 <td>0.2</td><td>0.2</td>
 </tr>
 <tr>
-<td>XbaⅠ</td><td>0.2</td><td>-</td>
+<td>XbaⅠ(μL)</td><td>0.2</td><td>-</td>
 </tr>
 <tr>
-<td>SpeⅠ</td><td>-</td><td>0.4</td>
+<td>SpeⅠ(μL)</td><td>-</td><td>0.4</td>
 </tr>
 <tr>
-<td>Buffer 2</td>
+<td>Buffer 2(μL)</td>
 <td>-</td><td>1.5</td>
 </tr>
 <tr>
-<td>Buffer 3</td>
+<td>Buffer 3(μL)</td>
 <td>2</td><td>-</td>
 </tr>
 <tr>
-<td>BSA</td>
+<td>BSA(μL)</td>
 <td>2</td><td>1.5</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>3.6</td><td>1.4</td></tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>20</td><td>15</td>
 </tr>
@@ Line 69: / Line 69: @@
 :--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
-::*Plac+RBS+RhlI Sample No.2~5
+::*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5
 ::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
@@ Line 78: / Line 78: @@
 </tr>
 <tr>
-<td>Sample DNA</td><td>1</td><td>3</td><td>3</td><td>1</td><td>3</td>
+<td>Sample DNA(μL)</td><td>1</td><td>3</td><td>3</td><td>1</td><td>3</td>
 </tr>
 <tr>
-<td>XbaⅠ</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
+<td>XbaⅠ(μL)</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 </tr>
 <tr>
-<td>SpeⅠ</td><td>-</td><td>0.1</td><td>-</td><td>-</td><td>0.1</td>
+<td>SpeⅠ(μL)</td><td>-</td><td>0.1</td><td>-</td><td>-</td><td>0.1</td>
 </tr>
 <tr>
-<td>Buffer 2</td>
+<td>Buffer 2(μL)</td>
 <td>0.9</td><td>0.8</td><td>0.9</td><td>0.9</td><td>0.8</td>
 </tr>
 <tr>
-<td>BSA</td>
+<td>BSA(μL)</td>
 <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>7</td><td>5</td><td>5</td><td>7</td><td>5</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
 </tr>
@@ Line 113: / Line 113: @@
 <tr>
 <td width="257">Sample No.</td>
-<td>Plac+RBS+RhlI Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①②
+<td>[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①②
 </td><td>Single & Double Digestion</td>
 </tr>
 <tr>
-<td>Sample DNA</td>
+<td>Sample DNA(μL)</td>
 <td>1</td><td>10</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(μL)</td>
 <td>1</td><td>2</td></tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>4</td><td>-</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>6</td><td>12</td>
 </tr>
 </table>
 :From Left
-::*Single Digestion Plac+RBS+RhlI 2~5
+::*Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
 ::*Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
-::*Double Digestion Plac+RBS+RhlI 2~5
+::*Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
 ::*Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
 ::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②