Team:Warsaw/Calendar-Main/10 September 2008

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<ol><li>Inoculation of all OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various cocnentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).</li>
<ol><li>Inoculation of all OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various cocnentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).</li>
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<li><p>Inoculation of OmpA_A_alfa, Omp_A_omega, Omp_Z_omega, Omp_alfa and Omp_omega to LB + 0.25 mM IPTG and kanamycin.</li>
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<li>Measurement of growth after 6 hours.</li></ol>
<li>Measurement of growth after 6 hours.</li></ol>

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Tests for ampicillin resistance given by protein added to medium

Piotr, Emila

  1. Inoculation of all OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various cocnentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).

  2. Inoculation of OmpA_A_alfa, Omp_A_omega, Omp_Z_omega, Omp_alfa and Omp_omega to LB + 0.25 mM IPTG and kanamycin.

  3. Measurement of growth after 6 hours.
Tabela z wynikami z exela- minimalna ilość biąłka do porzywki (ilość bilałka dodaną do porztwki przeliczono na nanomole białka- tu dać ile lażde z białek ma masy molowej):

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