Team background

This project was done by the NTU@iGEM team consisting of six undergraduate students during the period of May to July 2008. In addition, this project was also assisted by a number of graduate students and faculty advisors whom have given us valued advices and help throughout the project. For more details of those have helped us in our project, please kindly refer to the [Acknowledgements]. Students Darius Aw Kang Lie (Team Leader) Ng Zhen Fu (Modelling & Simulations Manager) Nguyen Xuan Hung (Information Technology Manager) Wong Choon Kit (Lab Manager) Lim Min-Lin (Business Manager) Yu Chin Chong (Treasurer and Characterization Manager) Faculty Advisors [http://www.ntu.edu.sg/scbe/CBE/FacultyPP/Profile_ProfChing.htm Prof Ching Chi Bun] [http://www.ntu.edu.sg/scbe/bioe/FacultyPP/Profile_CLPoh.htm Asst.Prof Poh Chueh Loo] [http://www.ntu.edu.sg/scbe/cbe/FacultyPP/Profile_Matthew.htm Asst.Prof Matthew Chang] We would like to extend our special thanks to [http://www3.ntu.edu.sg/SCBE/cbe/FacultyPP/Profile_William.htm A/Prof William Chen] for his guidance and help rendered to the iGEM team. We would also like to thank Dr Tan Tuan Lin for guiding the team and providing valuable insights for the experiments. Without their expertise and time, the project will not have gone this far in such a short time. Student Helper Lu Chao (Year 4 Bio-Engineering Student) Email all: ntu.igem@gmail.com

Our team logo ‎ Our team members (left to right): Yu Chin Chong, Wong Choon Kit, Ng Zhen Fu, Nguyen Xuan Hung, Lim Min-Lin, Darius Aw Kang Lie,

Project introduction

Our group has decided to engineer E.coli as a carrier for colicin E7 to deal with the pathogenic strain of Ecoli known as Escherichia coli O157:H7. Escherichia coli O157:H7 is an enterohemorrhagic strain that can cause bloody diarrhea and kidney failure. Current methods of cure for the infection include blood transfusion and dialysis. Currently no antibiotic is able to kill it.

Our approach is to use Colicin E7 to kill this harmful strain of bacteria. We intend to use our very own genetically engineered E.coli as the carrier of the colicin. We also wish for our bacteria to able to detect the presence of blood and the bacteria before it releases the colicins to eradicate the pathogen.

Our aim would be to control the level of colicin production in our bacteria. Our engineered bacteria should also be able to be accurate in its detection.

For any comments or enquiries, contact us at ntu.igem@gmail.com

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