Team:UC Berkeley/GatewayOverview

From 2008.igem.org

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(New page: In an effort to assist in the automation of synthetic biology, we investigated methods to simplify liquid handling so that it can more readily be performed by robots. We have accomplished ...)
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In an effort to assist in the automation of synthetic biology, we investigated methods to simplify liquid handling so that it can more readily be performed by robots. We have accomplished this by genetically encoding many of the required steps into the ''E. coli'', thereby allowing them to perform the required protocols.
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The reactions required to perform each step of layered assembly can be outsourced to engineered E. coli. For example, to move a Biobrick part from an entry vector to an assembly vector usually requires digestion with the appropiate enzymes, gel purifying the correct fragment, then ligating the part with the assembly vector backbone. Using an E.coli with our Gateway device, this is reduced to just transforming the entry vector with the Biobrick part, lysing with the self-lysis device, and then using the lysate in a transformation. A protocol that cannot be easily automated is thus reduced to a series of liquid transfers and heating/cooling steps.
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Our efforts to optimize synthetic biology protocols centered around the scheme for layered assembly. This involves the creation of biobrick parts in an entry vector, their transfer into double antibiotic assembly vectors, and subsequent assembly steps to connect various parts. After all of the assembly steps are complete, the desired composite parts can then be transferred into the destination vector of choice.
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Revision as of 06:53, 25 October 2008

The reactions required to perform each step of layered assembly can be outsourced to engineered E. coli. For example, to move a Biobrick part from an entry vector to an assembly vector usually requires digestion with the appropiate enzymes, gel purifying the correct fragment, then ligating the part with the assembly vector backbone. Using an E.coli with our Gateway device, this is reduced to just transforming the entry vector with the Biobrick part, lysing with the self-lysis device, and then using the lysate in a transformation. A protocol that cannot be easily automated is thus reduced to a series of liquid transfers and heating/cooling steps.

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