Team:Warsaw/Calendar-Main/1 August 2008

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<h4>Michał K.</h4>
<h4>Michał K.</h4>
<ol>
<ol>
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<li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-deltaA-alpha>pACYC177+OmpA_alpha + &Delta;A</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BOmpA-deltaA-omega>pACYC177+OmpA_omega + &Delta;A</a> colonies of tranformnts.</li>
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<li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-deltaA-alpha>pACYC177+OmpA_alpha + &Delta;A</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BOmpA-deltaA-omega>pACYC177+OmpA_omega + &Delta;A</a> colonies of tranformants.</li>
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<li> Gel electrphoresis of PCR products. </li>
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<li> Gel electrophoresis of PCR products (Fig. 1.).  </li>
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</ol>
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<img src="https://static.igem.org/mediawiki/2008/7/7c/Cos.jpg" width=300/> <var><b>Fig. 1. Colony PCR to find proper clones of pACYC177_OmpA_alpha+deltaA and pACYC177_OmpA_omega+deltaA</b><br>
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Upper gel:<br>
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1. Marker<br>
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2-13. colony PCR products to find proper clone of pACYC177_OmpA_alpha+deltaA <br>
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Lower gel:<br>
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1. Marker<br>
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2-11. colony PCR products to find proper clone of pACYC177_OmpA_omega+deltaA<br>
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12. positive control - pACYC_OmpA_A_alpha<br>
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13. negative control - pACYC_OmpA_Z_alpha<br></var>

Revision as of 23:45, 25 October 2008

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Cloning of truncated fragment of protein A

Michał K.

  1. Colony PCR on pACYC177+OmpA_alpha + ΔA and pACYC177+OmpA_omega + ΔA colonies of tranformants.
  2. Gel electrophoresis of PCR products (Fig. 1.).
Fig. 1. Colony PCR to find proper clones of pACYC177_OmpA_alpha+deltaA and pACYC177_OmpA_omega+deltaA
Upper gel:
1. Marker
2-13. colony PCR products to find proper clone of pACYC177_OmpA_alpha+deltaA
Lower gel:
1. Marker
2-11. colony PCR products to find proper clone of pACYC177_OmpA_omega+deltaA
12. positive control - pACYC_OmpA_A_alpha
13. negative control - pACYC_OmpA_Z_alpha



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