Team:Brown/Notebook/Protocols
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#To elute DNA, place QIAprep column in clean 1.5 mL centrifuge tube. Add 50 microliters EB or water to center of each QIAprep spin column, making sure to pipette EB right onto the cotton plug. Let stand for 1 minutes. Centrifuge for 1 minute. | #To elute DNA, place QIAprep column in clean 1.5 mL centrifuge tube. Add 50 microliters EB or water to center of each QIAprep spin column, making sure to pipette EB right onto the cotton plug. Let stand for 1 minutes. Centrifuge for 1 minute. | ||
- | ==Gel Electrophoresis== | + | ===Gel Electrophoresis=== |
*Gel Electrophoresis is primarily used to determine the length of a fragment of DNA. DNA Ladders are run beside experimental fragments as points of reference. A DNA Ladder contains all different lengths of DNA that when run out on a gel, separate to reveal the number of base pairs in each gene. | *Gel Electrophoresis is primarily used to determine the length of a fragment of DNA. DNA Ladders are run beside experimental fragments as points of reference. A DNA Ladder contains all different lengths of DNA that when run out on a gel, separate to reveal the number of base pairs in each gene. | ||
#Prepare the casting gel: 1% agarose gel - 1) 0.5 g agarose 2) 50 mL TBE + SYBR safe. | #Prepare the casting gel: 1% agarose gel - 1) 0.5 g agarose 2) 50 mL TBE + SYBR safe. |
Revision as of 14:43, 25 October 2008
ProtocolsMiniprep Prodedure
Gel Electrophoresis
Ligation Reaction Procedure
Transformation Procedure
Ethanol Precipitation
Gel Extraction
The contents of the tube should be yellow after incubation.
Calf CIP - Calf Intestinal Alkaline Phosphatase
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