Team:Warsaw/Calendar-Main/10 September 2008

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<img src="https://static.igem.org/mediawiki/2008/6/65/Wykres-piotr.jpg">
<img src="https://static.igem.org/mediawiki/2008/6/65/Wykres-piotr.jpg">
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<var>Bacterial growth depending on the amount of prey protein added to the medium. The amount is expressed in picomoles. Z_omega: 20323.75 Da, Z_alpha: 28734.24 Da, A_alpha: 37052.21 Da. </var>
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<var>Bacterial growth depending on the amount of prey protein added to the medium. The amount is expressed in picomoles per mL of medium. Z_omega: 20323.75 Da, Z_alpha: 28734.24 Da, A_alpha: 37052.21 Da. </var>
<p>Z_omega was added to bacteria culture expressing <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a><br>
<p>Z_omega was added to bacteria culture expressing <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a><br>

Revision as of 20:56, 25 October 2008

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Tests for ampicillin resistance given by protein added to medium

Piotr, Emila

  1. Inoculation of all pACYC177+OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various cocnentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).

  2. Inoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_A_omega, pACYC177+OmpA_Z_omega, pACYC177+OmpA_alpha and pACYC177+OmpA_omega to LB + 0.25 mM IPTG and kanamycin.

  3. Measurement of growth after 6 hours.
Bacterial growth depending on the amount of prey protein added to the medium. The amount is expressed in picomoles per mL of medium. Z_omega: 20323.75 Da, Z_alpha: 28734.24 Da, A_alpha: 37052.21 Da.

Z_omega was added to bacteria culture expressing pACYC177+OmpA_A_alpha
Z_alpha was added to bacteria culture expressing pACYC177+OmpA_A_omega.
A_alpha was added to bacteria culture expressing pACYC177+OmpA_Z_omega.

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