"
Team:Montreal/DNA Agarose Gel
From 2008.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | {| style="color:#ffffff;background-color:#cd0000;" cellpadding="3" cellspacing="5" border="2" bordercolor="#ffffff" width="75%" align="center" | ||
| + | !align="center"|[[Team:Montreal|Home]] | ||
| + | !align="center"|[[Team:Montreal/Team|The Team]] | ||
| + | !align="center"|[[Team:Montreal/Project|The Project]] | ||
| + | !align="center"|[[Team:Montreal/Parts|Parts Submitted to the Registry]] | ||
| + | !align="center"|[[Team:Montreal/Modeling|Modeling]] | ||
| + | !align="center"|[[Team:Montreal/Notebook|Notebook]] | ||
| + | |} | ||
| + | |||
| + | ==='''Procedure'''=== | ||
| + | |||
1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder. | 1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder. | ||
Revision as of 23:37, 16 June 2008
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook |
|---|
Procedure
1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder.
2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling).
3. Add 1.5µL of Ethidium Bromide and swirl until a fine mixture is seen.
4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends.
5. Wait for the gel to solidify over time.
6. Once hardened, remove the comb and load the gel into the electrophoresis box.
