Team:Warsaw/Calendar-Main/1 August 2008

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Cloning of truncated fragment of protein A

Michał K.

Colony PCR on pACYC177+OmpA_alpha + ΔA and pACYC177+OmpA_omega + ΔA colonies of tranformants.
Gel electrophoresis of PCR products Fig. 1.

Fig. 1. Colony PCR to find proper clones of pACYC177_OmpA_alpha+deltaA and pACYC177_OmpA_omega+deltaA Upper gel: 1. Marker 2-13. colony PCR products to find proper clone of pACYC177_OmpA_alpha+deltaA Lower gel: 1. Marker 2-11. colony PCR products to find proper clone of pACYC177_OmpA_omega+deltaA 12. positive control - pACYC_OmpA_A_alpha 13. negative control - pACYC_OmpA_Z_alpha

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 <ol>
 <li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-deltaA-alpha>pACYC177+OmpA_alpha + &Delta;A</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BOmpA-deltaA-omega>pACYC177+OmpA_omega + &Delta;A</a> colonies of tranformants.</li>
-<li> Gel electrophoresis of PCR products (Fig. 1.).  </li>
+<li> Gel electrophoresis of PCR products <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/1_August_2008#fig1">Fig. 1</a>.  </li>
 </ol>
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 <br>
-<img src="https://static.igem.org/mediawiki/2008/7/7c/Cos.jpg" width=300/> <var><b>Fig. 1. Colony PCR to find proper clones of pACYC177_OmpA_alpha+deltaA and pACYC177_OmpA_omega+deltaA</b><br>
+<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/7/7c/Cos.jpg" width=300/></a> <var><b>Fig. 1. Colony PCR to find proper clones of pACYC177_OmpA_alpha+deltaA and pACYC177_OmpA_omega+deltaA</b><br>
 Upper gel:<br>
 . Marker<br>