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Team:Heidelberg/Notebook/Sensing Group/Notebook/1stweek
From 2008.igem.org
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== Monday, 08/04/2008 == | == Monday, 08/04/2008 == | ||
| + | |||
| + | === Preparations === | ||
| + | Preparations were done at the end of the week before | ||
| + | * Transformation of pDK48 and pTrc99a in ''E. coli'' DH5a (0.5 µl plasmid-DNA + 50 µl competent cells) | ||
| + | * Glycerol-stock of ''Vibrio harveyi'' BB120, BB886, mm30, BB178, BB125 | ||
| + | * picked pTrc99a and pDK48 cultures and inoculated in 3 ml LB overnight | ||
| + | * ''V. harveyi'' cultured on LB+-Agar plate | ||
| + | * MiniPrep of pTrc99a and pDK48 from DHha | ||
| + | |||
| + | === Cloning of LuxP === | ||
| + | * PCR of LuxP from ''V. harveyi'' colony | ||
| + | * | ||
== Tuesday, 08/05/2008 == | == Tuesday, 08/05/2008 == | ||
Revision as of 09:32, 26 October 2008
Contents |
Monday, 08/04/2008
Preparations
Preparations were done at the end of the week before
- Transformation of pDK48 and pTrc99a in E. coli DH5a (0.5 µl plasmid-DNA + 50 µl competent cells)
- Glycerol-stock of Vibrio harveyi BB120, BB886, mm30, BB178, BB125
- picked pTrc99a and pDK48 cultures and inoculated in 3 ml LB overnight
- V. harveyi cultured on LB+-Agar plate
- MiniPrep of pTrc99a and pDK48 from DHha
Cloning of LuxP
- PCR of LuxP from V. harveyi colony
