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Team:Heidelberg/Notebook/Sensing Group/Notebook/3rdweek
From 2008.igem.org
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* Miniprep of pDK48 and LuxS | * Miniprep of pDK48 and LuxS | ||
* gel of Fusion constructs and gelextraction of pDK48 from yesterday | * gel of Fusion constructs and gelextraction of pDK48 from yesterday | ||
| + | * afterwards another gel was run to purify the Fusion constructs via gelextraction | ||
[[Image:HD 080819-Fusion PCR pDK48-Digestion.png|center|thumb|500px|Fusion PCR and pDK48 gel extraction]] | [[Image:HD 080819-Fusion PCR pDK48-Digestion.png|center|thumb|500px|Fusion PCR and pDK48 gel extraction]] | ||
Revision as of 14:27, 26 October 2008
Contents |
Monday, 08/18/2008
- PCR for LuxQ-1 and LuxQ-2 for Fusion constructs. Different Mg concentrations between 1-4 % were used. For LuxQ-2 another PCR was run using as template V. harveyi colonies
- Fusion-PCR for Fusion-1 and Fusion-2, each 4 tubes (2 µl Tar, 1 µl LuxQ)
- preparation of O/N culture of LuxS and pDK48 transformed cells for subsequent miniprep
- digestion of pDK48 with NcoI/NdeI
- digestion of pTrc99alpha and LuxQ (with BamHI and NcoI) and gelextraction
Tuesday, 08/19/2008
- Miniprep of pDK48 and LuxS
- gel of Fusion constructs and gelextraction of pDK48 from yesterday
- afterwards another gel was run to purify the Fusion constructs via gelextraction
