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Team:Heidelberg/Notebook/Sensing Group/Notebook/3rdweek
From 2008.igem.org
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* gel of Fusion constructs and gelextraction of pDK48 from yesterday | * gel of Fusion constructs and gelextraction of pDK48 from yesterday | ||
* afterwards another gel was run to purify the Fusion constructs via gelextraction | * afterwards another gel was run to purify the Fusion constructs via gelextraction | ||
| - | [[Image:HD 080819-Fusion PCR pDK48-Digestion.png|center|thumb|500px|Fusion | + | [[Image:HD 080819-Fusion PCR pDK48-Digestion.png|center|thumb|500px|Fusion constructs and pDK48 gel extraction]] |
== Wednesday, 08/20/2008 - Friday, 08/22/2008 == | == Wednesday, 08/20/2008 - Friday, 08/22/2008 == | ||
Revision as of 20:07, 26 October 2008
Monday, 08/18/2008
- PCR for LuxQ-1 and LuxQ-2 for Fusion constructs. Different Mg concentrations between 1-4 % were used. For LuxQ-2 another PCR was run using as template V. harveyi colonies
- Fusion-PCR for Fusion-1 and Fusion-2, each 4 tubes (2 µl Tar, 1 µl LuxQ)
- preparation of O/N culture of LuxS and pDK48 transformed cells for subsequent miniprep
- digestion of pDK48 with NcoI/NdeI
- digestion of pTrc99alpha and LuxQ (with BamHI and NcoI) and gelextraction
Tuesday, 08/19/2008
- Miniprep of pDK48 and LuxS
- gel of Fusion constructs and gelextraction of pDK48 from yesterday
- afterwards another gel was run to purify the Fusion constructs via gelextraction
Wednesday, 08/20/2008 - Friday, 08/22/2008
- nothing important to report
