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Team:Warsaw/Calendar-Main/16 July 2008
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a>). </li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a>). </li> | ||
<li> Control | <li> Control | ||
| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer) <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_July_2008#fig1">Fig. 1</a></li> <li> Gel electrophoresis - both checked colones are good .</li> | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer) (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_July_2008#fig1">Fig. 1.</a>).</li> <li> Gel electrophoresis - both checked colones are good .</li> |
</ol></p> | </ol></p> | ||
| - | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/a/ac/Dwa.jpg" width=300/></a> <var><b>Fig. 1. Control SacI/BamHI digests of isolated plasmids | + | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/a/ac/Dwa.jpg" width=300/></a> <var><b>Fig. 1. </b>Control SacI/BamHI digests of isolated plasmids<br> |
1. Marker<br> | 1. Marker<br> | ||
2-3. digested plasmids pACYC177+OmpA_Z_omega <br></var> | 2-3. digested plasmids pACYC177+OmpA_Z_omega <br></var> | ||
Revision as of 17:49, 26 October 2008
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Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, MarcinWe have massive outbreak of some virulent bacteriophage strain in our lab. All our current E. coli cultures are gone :-(. Until we get rid of the phage there will be no microbiological work in our lab. Sorry. Cloning of protein Z DNA to OmpA constructsMichał K.
 Fig. 1. Control SacI/BamHI digests of isolated plasmids1. Marker 2-3. digested plasmids pACYC177+OmpA_Z_omega |
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