"
Team:Warsaw/Calendar-Main/1 August 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
|||
| Line 5: | Line 5: | ||
<html> | <html> | ||
| - | <h3> Cloning of truncated fragment of protein A</h3> | + | <h3> Cloning of truncated fragment of protein A (ΔA)</h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<ol> | <ol> | ||
| Line 15: | Line 15: | ||
<br> | <br> | ||
| - | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/7/7c/Cos.jpg" width=300/></a> <var><b>Fig. 1.</b> Colony PCR to find proper clones of pACYC177_OmpA_alpha+ | + | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/7/7c/Cos.jpg" width=300/></a> <var><b>Fig. 1.</b> Colony PCR to find proper clones of pACYC177_OmpA_alpha+ΔA and pACYC177_OmpA_omega+ΔA<br> |
Upper gel:<br> | Upper gel:<br> | ||
1. Marker<br> | 1. Marker<br> | ||
| - | 2-13. colony PCR products to find proper clone of pACYC177_OmpA_alpha+ | + | 2-13. colony PCR products to find proper clone of pACYC177_OmpA_alpha+ΔA <br> |
Lower gel:<br> | Lower gel:<br> | ||
1. Marker<br> | 1. Marker<br> | ||
| - | 2-11. colony PCR products to find proper clone of pACYC177_OmpA_omega+ | + | 2-11. colony PCR products to find proper clone of pACYC177_OmpA_omega+ΔA<br> |
12. positive control - pACYC_OmpA_A_alpha<br> | 12. positive control - pACYC_OmpA_A_alpha<br> | ||
13. negative control - pACYC_OmpA_Z_alpha<br></var> | 13. negative control - pACYC_OmpA_Z_alpha<br></var> | ||
Latest revision as of 18:42, 26 October 2008
 |
|
||||||||
 |
|
||||||||
Cloning of truncated fragment of protein A (ΔA)Michał K.
 Fig. 1. Colony PCR to find proper clones of pACYC177_OmpA_alpha+ΔA and pACYC177_OmpA_omega+ΔAUpper gel: 1. Marker 2-13. colony PCR products to find proper clone of pACYC177_OmpA_alpha+ΔA Lower gel: 1. Marker 2-11. colony PCR products to find proper clone of pACYC177_OmpA_omega+ΔA 12. positive control - pACYC_OmpA_A_alpha 13. negative control - pACYC_OmpA_Z_alpha
|
|
