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Team:Rice University/Notebook/9 June 2008
From 2008.igem.org
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****Tm = 81.7 C TaOpt: 60.4 C GC: 56.3 | ****Tm = 81.7 C TaOpt: 60.4 C GC: 56.3 | ||
****area of interest = 20833 | ****area of interest = 20833 | ||
| - | ****sense primer: GAGACGAATGCCAGGTCATCTGAAA | + | ****sense primer: GAGACGAATGCCAGGTCATCTGAAA <---------------primer name: ATTP L |
****length: 25 Tm = 60.3 C GC = 48.0 | ****length: 25 Tm = 60.3 C GC = 48.0 | ||
| - | ****antisense primer: AAATCTGGATCATTCCCGAGCGCTG | + | ****antisense primer: AAATCTGGATCATTCCCGAGCGCTG <-----------primer name: ATTP R |
****length: 25 Tm = 64.9 C GC = 52.0 | ****length: 25 Tm = 64.9 C GC = 52.0 | ||
***Primer Set 2: | ***Primer Set 2: | ||
| Line 16: | Line 16: | ||
****Tm = 70.7 C TaOpt: 51.4 C GC: 31.7 | ****Tm = 70.7 C TaOpt: 51.4 C GC: 31.7 | ||
****area of interest = 23539 | ****area of interest = 23539 | ||
| - | ****sense primer: ATCACATCGTCACCCATTGGATTGT | + | ****sense primer: ATCACATCGTCACCCATTGGATTGT <---------------primer name: STF L |
****length: 25 Tm = 60.1 C GC = 44.0 | ****length: 25 Tm = 60.1 C GC = 44.0 | ||
| - | ****antisense primer: CGATTTAGAAATGTATAGCGAGGCA | + | ****antisense primer: CGATTTAGAAATGTATAGCGAGGCA <-----------primer name: STF R |
****length: 25 Tm = 55.9 C GC = 40.0 | ****length: 25 Tm = 55.9 C GC = 40.0 | ||
Revision as of 19:46, 18 June 2008
Monday 9 June
- Selim Sheikh:
- Designed 2 sets of sequencing primers (using Vector NTI Advance 10 http://www.invitrogen.com/site/us/en/home/LINNEA-Online-Guides/LINNEA-Communities/Vector-NTI-Community/Sequence-analysis-and-data-management-software-for-PCs.html) to be used in PCR of lambda DNA to amplify target regions:
- Primer Set 1:
- product of length 538
- contains region of the molecule from 20481 to 21018
- Tm = 81.7 C TaOpt: 60.4 C GC: 56.3
- area of interest = 20833
- sense primer: GAGACGAATGCCAGGTCATCTGAAA <---------------primer name: ATTP L
- length: 25 Tm = 60.3 C GC = 48.0
- antisense primer: AAATCTGGATCATTCCCGAGCGCTG <-----------primer name: ATTP R
- length: 25 Tm = 64.9 C GC = 52.0
- Primer Set 2:
- product of length 309
- contains region of the molecule from 23341 to 23649
- Tm = 70.7 C TaOpt: 51.4 C GC: 31.7
- area of interest = 23539
- sense primer: ATCACATCGTCACCCATTGGATTGT <---------------primer name: STF L
- length: 25 Tm = 60.1 C GC = 44.0
- antisense primer: CGATTTAGAAATGTATAGCGAGGCA <-----------primer name: STF R
- length: 25 Tm = 55.9 C GC = 40.0
- Primer Set 1:
- Designed 2 sets of sequencing primers (using Vector NTI Advance 10 http://www.invitrogen.com/site/us/en/home/LINNEA-Online-Guides/LINNEA-Communities/Vector-NTI-Community/Sequence-analysis-and-data-management-software-for-PCs.html) to be used in PCR of lambda DNA to amplify target regions:
