Revision as of 17:24, 19 June 2008

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Things we did today

KS

cancelled because there are better ways to test of our plasmid prep worked, we'll digest and run it on gel to verify

Grace

**Colony Count of Transformants**
Plasmid	Dilution	Colony Forming Units on Plate	Number of Transformed Cells per mL
pBluescript	1:1	79	3950
	1:10	12	6000
	1:100	1	5000
BBa_B0024	1:1	0	n/a
BBa_B0025	1:1	1	50
BBa_E0240	1:1	0	n/a

"What's that band-aid for on your hand"-NW

"I was testing out this bottle of anesthetics then pricked myself with needles"-KS

"Umm OK..."-NW

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 6: / Line 6: @@
 :'''KS'''
 ''cancelled because there are better ways to test of our plasmid prep worked, we'll digest and run it on gel to verify''
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
-:* e.g. worked on &lt;blah experiment link&gt;, PCR, ran gel
+=== Counted transformation efficiency of new competent cells===
+: '''Grace'''
+{| border="1"
+|+ '''Colony Count of Transformants'''
+!Plasmid
+!Dilution
+!Colony Forming Units on Plate
+!Number of Transformed Cells per mL
+|-
+| rowspan="3" align="center"|pBluescript||align="center"| 1:1 || align="center"|79|| align="center"|3950
+|-
+|  align="center"|1:10|| align="center"|12|| align="center"|6000
+|-
+|  align="center"|1:100|| align="center"|1||align="center"|5000
+|-
+| " align="center"|BBa_B0024||align="center"|1:1||align="center"|0||align="center"|n/a
+|-
+| align="center"|BBa_B0025||align="center"|1:1|| align="center"|1|| align="center"|50
+|-
+| align="center"|BBa_E0240||align="center"|1:1|| align="center"|0 ||align="center"|n/a
+|}
 = Discussion =