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Alberta NINT/14 May 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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lab work (SD): | lab work (SD): | ||
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Incubated at 37 C for 2 hrs. Run on 2% agarose gel. | Incubated at 37 C for 2 hrs. Run on 2% agarose gel. | ||
Expected fragment lengths: R0010 200 bp, R0011 55 bp, E0433 533 bp, pSB1A2 ~2 kbp. | Expected fragment lengths: R0010 200 bp, R0011 55 bp, E0433 533 bp, pSB1A2 ~2 kbp. | ||
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| + | [[Alberta_NINT/15_May_2008 | Next entry >]] | ||
Revision as of 20:56, 19 June 2008
lab work (SD):
Isolated plasmid DNA from culture tubes using QIAprep miniprep standard protocol.
Digested DNA with EcoRI + PstI.
Incubated at 37 C for 2 hrs. Run on 2% agarose gel.
Expected fragment lengths: R0010 200 bp, R0011 55 bp, E0433 533 bp, pSB1A2 ~2 kbp.
