Team:Brown/Project/Overview

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*Brown University’s Team Resistance aims to create a Biosensor that utilizes a novel method to detect the presence of a toxin in a solution of bacteria. A gene cassette coding for cell lysis in E. Coli will be placed under the control of an inducible promoter such as a pBAD promoter.  Once cell lysis has occurred, the charged intracellular contents of the cells will become part of the extra-cellular solution thus decreasing the solution’s resistance and indicating the presence of a toxin or other inducer.
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*Brown University’s Team Resistance aims to create a Biosensor that utilizes a novel method to detect the presence of a toxin in a solution of bacteria. A gene cassette coding for cell lysis in E. Coli will be placed under the control of an inducible promoter such as a pBAD promoter.  Once cell lysis has occurred, the charged intracellular contents of the cells will become part of the extra-cellular solution thus increasing the solution’s conductance and indicating the presence of a toxin or other inducer.

Revision as of 00:22, 28 October 2008




  • Brown University’s Team Resistance aims to create a Biosensor that utilizes a novel method to detect the presence of a toxin in a solution of bacteria. A gene cassette coding for cell lysis in E. Coli will be placed under the control of an inducible promoter such as a pBAD promoter. Once cell lysis has occurred, the charged intracellular contents of the cells will become part of the extra-cellular solution thus increasing the solution’s conductance and indicating the presence of a toxin or other inducer.


Strategy

Guidelines for an ideal biosensor:

Biological

  • Uses minimal biological machinery
  • Direct induction of system by inducer creates a sensitive system
  • Versatile construct can measure different substances/toxins, i.e. arabinose (proof of concept), lead, mercury, arsenic

Engineering

  • Compact system for sample analysis
  • Economically feasible
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