Alberta NINT/14 May 2008
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Incubated at 37 C for 2 hrs. Run on 2% agarose gel. | Incubated at 37 C for 2 hrs. Run on 2% agarose gel. | ||
Expected fragment lengths: R0010 200 bp, R0011 55 bp, E0433 533 bp, pSB1A2 ~2 kbp. | Expected fragment lengths: R0010 200 bp, R0011 55 bp, E0433 533 bp, pSB1A2 ~2 kbp. | ||
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+ | [[Image:UVP00294.jpg]] | ||
[[Alberta_NINT/15_May_2008 | Next entry >]] | [[Alberta_NINT/15_May_2008 | Next entry >]] |
Revision as of 20:43, 7 July 2008
lab work (SD):
Isolated plasmid DNA from culture tubes using QIAprep miniprep standard protocol. Digested DNA with EcoRI + PstI. Incubated at 37 C for 2 hrs. Run on 2% agarose gel. Expected fragment lengths: R0010 200 bp, R0011 55 bp, E0433 533 bp, pSB1A2 ~2 kbp.